窄带薄层色谱-表面增强拉曼光谱联用法的建立及应用

建立窄带薄层色谱-表面增强拉曼光谱(narrow-band TLC-SERS)分析方法来改善传统薄层色谱-表面增强拉曼光谱法(TLC-SERS)在色谱展开过程中斑点横向扩散以及由于滴加SERS基底而导致的斑点二次扩散的不足,进一步提高TLC-SERS方法的检测灵敏度。本文采用色谱层析硅胶GF254制备并优化出宽度为2 mm的窄带TLC板,待样品在窄带TLC板上分离后,在分离斑点表面喷洒银溶胶,然后对斑点进行SERS检测。结果表明,narrow-band TLC-SERS法具有简便、快速、灵敏的特点,不仅改善了传统TLC-SERS方法斑点横向扩散的不足,而且降低了固定相的用量,节约了成本,具有非常广阔的应用前景。

Abstract

The narrow-band thin layer chromatography combing with surface enhanced Raman spectroscopy(narrow-band TLC-SERS)method was established to further improve the sensitivity of conventional TLC-SERS method,which can restrain transverse diffusion of analytes in TLC development and overcame the second diffusion of analytes attributed to adding SERS-active substrate.In this work,the narrow-band TLC plate with width of 2 mm was prepared using the silica gel GF254as stationary phase.After the sample applied on the narrow-band TLC plate,the silver colloid was deposited on the surface of separated spots,and then the spots were detected by portable Raman spectrometer.The results indicate that the narrow-band TLC-SERS method is simple,rapid and sensitive.Furthermore,this method not only restrains the defect of the transverse diffusion of analytes in TLC development but also saves the cost.Thus,the narrow-band TLC-SERS method has good prospects for application.

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张彬彬, 史毅, 朱青霞, 陆峰. 窄带薄层色谱-表面增强拉曼光谱联用法的建立及应用[J]. 光散射学报, 2017, 29(2): 129. ZHANG Binbin, SHI Yi, ZHU Qingxia, LU Feng. Establishment and Application of Narrow-Band TLC-SERS[J]. The Journal of Light Scattering, 2017, 29(2): 129.

窄带薄层色谱-表面增强拉曼光谱联用法的建立及应用

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