不同产地茯苓皮药材红外光谱的识别

马芳; 张方; 汤进; 陈平; 陈建波; 周群; 孙素琴

doi:doi:10.3964/j.issn.1000-0593(2014)02-0376-05

光谱学与光谱分析, 2014, 34 (2): 376, 网络出版: 2015-01-13

不同产地茯苓皮药材红外光谱的识别

Analysis and Identification of Poria Cocos Peels Harvested form Different Producing Areas by FTIR and 2D-IR Correlation Spectroscopy

论文大纲

马芳 ^1,*张方 ¹汤进 ¹陈平 ¹陈建波 ²周群 ²孙素琴 ²

作者单位

¹ 武汉轻工大学生物与制药工程学院，湖北　武汉430023

² 清华大学化学系，北京100084

摘要

采用红外光谱、二阶导数光谱和二维相关红外光谱对大别山地区和云南省两大道地主产区的茯苓皮进行鉴别分析。结果表明，不同产地茯苓皮的红外光谱都有1　149， 1　079和1　036 cm-1等表征糖类成分的特征吸收峰，其中大别山产区（湖北省、安徽省）的茯苓皮中1　619， 1　315和780 cm-1等表征草酸钙的特征吸收峰比较显著，云南产区的茯苓皮中797， 779， 537和470 cm-1等表征硅酸盐的特征吸收峰更加明显。比较450～1 650 cm-1波数范围内的二阶导数红外光谱可知，两个产区茯苓皮所含糖类物质有所不同。在二维相关红外光谱中，不同产地茯苓皮所含糖类物质的差异更加直观显著。红外光谱法直观、简单、方便、快速，可以作为鉴别不同产地茯苓皮，诠释产地与药效之间关联性的一种有效准确的新方法。

Abstract

Different geographical regions of traditional Chinese medicine (TCM), its chemical composition is different, the accumulation of drug and medicinal properties is different. The accurate identification and analysis of different production area of medicinal herbs is critical for the quality control and pharmacological research of TCM. In this paper, a tri-step infrared spectroscopy (Fourier transform infrared spectroscopy (FTIR) combined with second derivative spectra and two-dimensional correlation infrared spectroscopy (2D-COS) were employed to identify and analyze the main components of Hubei(HB), Anhui(AH), Yunnan(YN) genuine Poria Cocos peels. The emergence of several characteristic absorption peaks of carbohydrates including 1　149, 1　079 1　036 cm-1, peaks around 1　619, 1　315, 780 cm-1 belonged to calcium oxalate suggested that HB and AH Poria Cocos peels contained calcium oxalate, but peaks around 797, 779, 537, 470 cm-1 belonged to kaoline suggested that YN Poria Cocos peels contained kaoline. Their carbohydrates were different by comparing the second derivative infrared spectra in the range of 1　640～450 cm-1 and Yongping come from YN contains both calcium oxalate and kaoline. Furthermore, the above differences were visually validated by two-dimensional correlation spectroscopy (2D-COS). It was demonstrated that the Tri-step infrared spectroscopy were successfully applied to fast analyze and identify Poria Cocos peels from different geographical regions and subsequently would be applicable to explain the relevance of geographical regions and medicinal properties for the TCM.

参考文献

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马芳, 张方, 汤进, 陈平, 陈建波, 周群, 孙素琴. 不同产地茯苓皮药材红外光谱的识别[J]. 光谱学与光谱分析, 2014, 34(2): 376. MA Fang, ZHANG Fang, TANG Jin, CHEN Ping, CHEN Jian-bo, ZHOU Qun, SUN Su-qin. Analysis and Identification of Poria Cocos Peels Harvested form Different Producing Areas by FTIR and 2D-IR Correlation Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2014, 34(2): 376.

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