Author Affiliations
Abstract
1 Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences, No. 390, Qinghe Road, Jiading P. O. Box 800-211, Shanghai 201800, P. R. China
2 State Key Laboratory of High Field Laser Physics Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences, No. 390, Qinghe Road, Jiading P. O. Box 800-211, Shanghai 201800, P. R. China
Published 15 August 2012 Recently, we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules as fluorescent labels can enable optical resolutions far beyond the diffraction-limit. Here, we show that by combining the 4Pi microscopy and the novel fluorescent label, it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.
Fluorescent dye 4Pi microscopy far-field imaging super-resolution confocal Journal of Innovative Optical Health Sciences
2012, 5(3): 1250016