Photodynamic inactivation of microorganisms known as antibacterial photodynamic therapy (APDT) is one of the most promising and innovative approaches for the destruction of pathogenic microorganisms. Among the photosensitizers (PSs), compounds based on cationic porphyrins/metalloporphyrins are most successfully used to inactivate microorganisms. Series of meso-substituted cationic pyridylporphyrins and metalloporphyrins with various peripheral groups in the third and fourth positions of the pyrrole ring have been synthesized in Armenia. The aim of this work was to determine and test the most effective cationic porphyrins and metalloporphyrins with high photoactivity against Gram negative and Gram positive microorganisms. It was shown that the synthesized cationic pyridylporphyrins/metalloporphyrins exhibit a high degree of phototoxicity towards both types of bacteria, including the methicillinresistant S. aureus strain. Zinc complexes of porphyrins are more phototoxic than metal-free porphyrin analogs. The effectiveness of these Zn–metalloporphyrins on bacteria is consistent with the level of singlet oxygen generation. It was found that the high antibacterial activity of the studied cationic porphyrins/metalloporphyrins depends on four factors: the presence in the porphyrin macrocycle of a positive charge (+4), a central metal atom (Zn2tT and hydrophobic peripheral functional groups as well as high values of quantum yields of singlet oxygen. The results indicate that meso-substituted cationic pyridylporphyrins/metalloporphyrins can find wider application in photoinactivation of bacteria than anionic or neutral PSs usually used in APDT.

通过系统分析绿色植被在紫外可见波段光谱的产生机理, 得出绿峰和红边是由叶绿素产生的。 从叶绿素的结构上看, 它属于卟啉类化合物, 为了进一步探究红边产生的机理, 合成了四苯基卟啉、 四(4-甲氧基苯基)卟啉、 四(4-磺酸钠苯基)卟啉、 四吡啶基卟啉、 四甲基卟啉、 四(4-甲氧基苯基)卟啉合锌和四(4-甲氧基苯基)卟啉合铜, 并采用紫外可见光谱、 红外及核磁进行表征。 通过对其紫外可见光谱的系统分析, 提出“红边”不仅限于叶绿素, 而是卟啉类化合物特有的光谱特征, 红边是由卟啉环a2u(π)-eg(π*) 跃迁产生的Q带所致。 其位置不仅与卟啉的浓度相关, 与卟啉化合物外侧取代基类型也有关系, 金属卟啉对红边的位置影响较大。

Many up-to-date optical techniques have been developed and applied recently in clinical practice for obtaining qualitatively and quantitatively new data from the investigated lesions. Due to their high sensitivity in detection of small changes, these techniques are widely used for detection of early changes in biological tissues. Light-induced fluorescence spectroscopy (LIFS) is one of the most promising techniques for early detection of cutaneous neoplasia. Increasing number of recent publications have suggested that optical coherence tomography (OCT) also has potential for non-invasive diagnosis of skin cancer. This recent work is a part of clinical trial procedure for introduction of LIFS technique into the common medical practice in National Oncological Medical Center in Bulgaria for diagnosis of non-melanoma skin cancer. We focus our attention here on basal cell carcinoma lesions and their specific features revealed by LIFS and OCT analysis. In this paper we prove the efficiency of using the combined LIFS-OCT method in skin lesions studies by integrating the complimentary qualities of each particular technique. For LIFS measurements several excitation sources, each emitting at 365, 385 and 405 nm maxima are applied. An associated microspectrometer detects in vivo the fluorescence signals from human skin. The main spectral features of the lesions and normal skin are discussed and their possible origins are indicated. OCT images are used to evaluate the lesion thickness, structure and severity stage, when possible. The obtained results could be used to develop a more complete picture of optical properties of these widely spread skin disorders. At the same time, our studies show that the combined LIFS-OCT method could be introduced in clinical algorithms for early tumor detection and differentiation between normal/benign/malignant skin lesions.

建立了单光子光化烧孔的三能级模型,求解了反映质子转位变构机制的速率方程组,推导了成孔过程的动力学特性,获得了可与实验进行比较的解析解.用时域和频域两种方法测量了THP/PMMA和TMP/PMMA薄膜样品光谱烧孔的动力学过程,得到与理论分析一致的规律,并测得了有效成孔速率和成孔时间等参数.这些参数对于评价频域光存贮材料性能有一定意义.