三角褐指藻是生产生物柴油的优势藻种, 监测其光合和生长参数是利用它们生产生物柴油的关键环节。本研究通过细胞计数对细胞生长量进行监测, 利用多激发波长调制叶绿素荧光仪(Multicolor PAM)、NanoDrop紫外分光光度计与荧光分光光度计分别检测叶绿素荧光参数、培养基中硝酸盐含量、细胞叶绿素荧光强度与中性脂相对含量。结果显示细胞密度与叶绿素荧光强度只有在生长期才具有线性关系, 达到稳定期后, 叶绿素荧光强度显著性下降, 此时与细胞密度不成比例; PSII 最大光化学量子效率Fv/Fm与叶绿素荧光值比生长速率呈正相关, 在整个培养阶段Fv/Fm的变化趋势呈现两次上升与下降的过程; 光化学淬灭系数qL在培养过程中呈上升趋势, 而相对电子传递速率rETR与实际光合效率Y(II)均呈现先升后降的趋势; 培养基中硝酸盐含量消耗到一定程度后保持恒定, 不再降低; 细胞中性脂相对含量在藻细胞接种1天后稍下降, 随后持续上升, 并在培养基硝酸盐含量不再下降后继续增加。本研究中光合与生长参数测量得到的相关数据, 以及培养基硝酸盐含量和细胞中性脂相对含量测量方法的建立, 可以为三角褐指藻实验室培养及其代谢产物积累的研究提供必要的研究基础与检测手段。

Purpose: The examination of the effects of second generation lotrafilcon B silicone hydrogel (SiH) lenses on the cornea when worn for three years of daily wear. Material and Methods: 55 healthy patients were divided into two groups: current hydrogel contact lens wearers refitted with lotrafilcon B lenses (Group 1; 28 patients) and neophyte contact lens wearers (Group 2; 27 patients). Each patient's subjective eye comfort was measured with a self-administered questionnaire. The corneas were analyzed using contact specular microscope to measure corneal thickness and the endothelium before the SiH lenses were fitted after four weeks, one month, six months, one year, two years, and three years of lens wear. Results: Subjective complaints of patients in Group 1 were reduced; however patients in Group 2 experienced discomfort during the first two to four weeks of use. In Group 1, objective examinations identified a decrease in endothelial cell density. In Group 2, the endothelial cell density increased slightly in the first two years but decreased after three years. The results indicate that lotrafilcon B slows down the deleterious effects of contact lenses. The coefficient of variation significantly decreased after six months in Group 1 (0.47 vs 0.44; p = 0.049), whereas, in Group 2, the hexagonal cells improved significantly after one month (27.78 vs 28.25; p = 0.025). Conclusion: Based on the subjects involved and the period of time under examination, it can be concluded that high-Dk SiH lenses support the physiological metabolism and functions of the cornea by improving oxygen provision.