Vannhu Le 1,2,3,*
Author Affiliations
Abstract
1 Department of Optical Engineering, Le Quy Don Technical University, 236 Hoang Quoc Viet Street, Tu Liem District, Hanoi, Vietnam
2 State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China
3 Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
3D live imaging is important for better understanding of biological processes. To obtain biological dynamic process, high imaging speed is required. In order to improve speed in 3D live imaging, simultaneous imaging of multiple planes throughout a 3D volume has been proposed. However, a main disadvantage of this method is the cross-talk from neighboring imaging planes. In this paper, we propose an optimization method to suppress background from neighboring imaging planes. A D-aperture is used to generate multiple light sheets. An optimization method to suppress background is presented. The simulation results demonstrated that the proposed method can be used to suppress the effectiveness of background from neighboring light sheets.
Fluorescence microscopy imaging systems supper-resolution 
Journal of Innovative Optical Health Sciences
2019, 12(2): 1950004
作者单位
摘要
1 西安电子科技大学, 西安 710071
2 中国科学院光电研究院, 北京 100094
为了进一步提高超分辨卫星载荷光学系统的空间分辨能力, 需对系统杂散光进行抑制, 以得到准确的原始图像.通过光线追迹方式, 分析了系统杂散光, 确认了杂散光的来源.通过Tracepro定量化分析和结构有限元分析, 在限制范围内, 不断优化、改进遮光罩尺寸及挡光环位置, 得到光学系统视场外点源透射比均在10-3以下, 验证了该杂散光抑制方案的有效性, 为卡塞格林光学系统的工程应用提供参考.
光学设计 超分辨 卡塞格林光学系统 杂散光 点源透射比 遮光罩 Optical system design Supper-resolution Cassegrain optical system Stray light Point Sources Transmittance 
光子学报
2017, 46(11): 1122002

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