基于光谱成像技术的手术导航通过分析不同组织的光谱差异，能够有效识别各类组织，具有重要的应用价值，但目前光谱成像的采样速度和光谱图像的呈现方式极大地限制了其在临床中的应用。提出面向手术导航的增强现实计算光谱成像系统，通过使用RGB成像器件和多光谱单像素探测器，实现单次成像下的高质量光谱重构，极大地提升了成像速度和重构效率；采用主成分分析和光谱角制图提取光谱图像的有效信息，突出特征组织区域；展示了头戴式增强现实显示设备中光谱图像与实际手术区域融合后实现的图像增强效果。

Purpose: To introduce the application of intraoperative optical coherence tomography (iOCT) in pars plana vitrectomy (PPV) for various vitreoretinal diseases, and to report the 4-year assessment of feasibility and utility in Chinese population. Methods: Retrospective case series of patients who underwent PPV and iOCT scan at Eye Hospital of Wenzhou Medical College from January 2016 to January 2020. Clinical characteristics were documented before operation, and we intraoperatively recorded the time and results of iOCT scanning, specific surgical maneuvers performed, the consistency with the planned strategies before surgery, the type of OCT images obtained, and adverse events (AEs). The surgeon feedback was collected to evaluate the utility of iOCT during surgery. Results: In total 339 eyes successfully completed iOCT scan, with an average scanning time of 3.54 ± 2.3 min, including 59 cases of idiopathic macular hole (iMH), 134 cases of idiopathic epiretinal membrane (iERM), 33 cases of lamellar macular hole (LMH), 40 cases of high myopic maculopathy, 13 cases of vitreous macular traction (VMT), 60 cases of dense vitreous hemorrhage (VH). The iOCT findings were not consistent with examination under the operating microscope in 49 cases (14.5%), including 29 cases (8.6%) which changed the operation strategies during surgery. The Hole-door phenomenon arose in 20 cases (33.9%) of iMH and 3 cases (25%) of high myopic MH after ILMs peeling. Moreover, the residue ERM was observed in nine cases (6.7%) of iERM and two cases (14.3%) in high myopic ERM after ILMs peeling. Some new surgical methods could also be confirmed using iOCT. Conclusion: The application of iOCT has a significant clinical functionality in vitreoretinal surgery, providing the surgeon with a new surgical understanding, guiding the selection of a more reasonable operative procedure during surgery, predicting postoperative recovery and improving postoperative outcomes.

多光谱成像(MSI)融合了光谱技术与成像技术,可并行获取探测目标的光谱特征和空间信息。由于采用非侵入式的成像方式,该技术在生物医学领域有很多重要的应用。介绍了多光谱成像的基本原理与技术发展,并从病理研究、手术引导、生物识别等三个方面对其应用进行简要综述。

A technical feasibility of autofluorescence ductoscopy in breast milk ducts as blood vessels phantoms has been assessed as successful. Malignant tumor can be clearly identified through the milk ducts. We also present the operation principle as well as the preliminary experimental results of a new type of microsize multicore fiber that enables imaging through blood vessel phantoms. Imaging of a manipulated microwire through a drilled phantom is presented.

Limitations of cancer margin delineation and surgical guidance by means of autofluorescence imaging under conditions of laser ablation were investigated and preliminary results are presented. PinPointTM (Novadaq Technologies Inc., Canada) was used to capture digital images and Er:YAG laser (2.94μm, Glissando, WaveLightTM, Germany) was exploited to cause laser ablation on both normal and cancer sites of the specimen. It was shown that changes of the autofluorescence image after ablation extend beyond the actual sizes of the ablation loci. The tumor tissue after the laser ablation starts to emit fluorescent light within the green wavelength band (490–550nm) similar to normal tissue stating that the current technology of in-process tissue classification fails. However, when the autofluorescence was collected in the red range (600–750nm), then the abnormal/normal contrast was reduced, but still present even after the laser ablation. The present study highlights the importance of finding a proper technology for surgical navigation of cancer removal under conditions of high power effects in biological tissues.