为扣除溶剂或其他背景组分的干扰, 测量红外光谱时常常需要获得期望强度的高质量背景单光束谱。 通常, 实验上获得期望强度的背景谱是极其困难的。 为实现这一重要且十分困难的目标, 引进了杂化单光束谱的概念。 同一溶液但不同厚度的b1和b2的两样品的单光束谱分别为b1和b2, 则定义它们的线性组合α=αb1+(1－α)b2为杂化单光束谱, 其中α(0≤α≤1)称为组分因子。 调整组分因子α数值, 就可以精确调控杂化谱的强度。 在合适的条件下, 杂化谱α与厚度为b2－αb2+αb1的真实样品的光谱高度类似, 即b2－αb2+αb1≈α。 因此, 不用制备厚度为b2－αb2+αb1的样品, 其单光束谱可以用α来替代。 随着α变化, 可以得到不同的α, 厚度在b1和b2间的真实样品的单光束谱都可用相应的α来替代。 实验结果证实, 杂化谱提供了一种简单和易操作的扣除背景干扰的高效方法。

In order to compensate for solvent bands and other background effects, a successful infrared (IR) spectral measurement requires a quality single-beam background spectrum with a desired intensity. Usually, it is extremely difficult to bring a background component to the desired intensity in practice. In order to achieve this important but difficult goal, a hybrid single-beam spectrum α=αb1+(1－α)b2 is introduced as the combination of two single-beam spectra b1 and b2 from the same sample but with different pathlengths (b1 and b2), where α (0≤α≤1) is the component factor. Obviously, the intensity of the hybrid spectrum α can be controlled easily to the desired point by simply choosing an appropriate component factor. Under appropriate conditions, the hybrid spectrum α is very nearly identical to the single-beam spectrum obtained from the real sample with the pathlength of b2-αb2+αb1 and the minor spectral distortion of α can be dismissed. As a result, the single-beam spectrum of a real sample b with a pathlength of b2-αb2+αb1 can be represented by α and the real sample does not need to be prepared. Experimentally, the hybrid single-beam spectrum provides a very simple, robust and efficient method to overcome the interference from background samples of IR measurements and shows valuable potential in applications of this field.