用50 μW/cm2 UV-B连续辐射大豆愈伤组织16 h, 发现大豆愈伤组织的自发发光强度逐渐增长, 延迟发光呈现衰减的趋势。通过对延迟发光动力学过程的分析, 得到了UV-B辐射下大豆愈伤组织延迟发光的特征参数积分强度I(T)、初始光子数I0、相干时间τ和衰减参数β的变化规律。结果表明, 大豆愈伤组织延迟发光初始光子数I0、相干时间τ和衰减参数β随着UV-B辐射时间的延长逐渐减小, 延迟发光积分强度I(T)与初始光子数I0成极好的正相关。根据生物超弱光子辐射的意义定义了描述细胞生命状态有序程度的序参量, 发现在50 μW/cm2 UV-B连续辐射下, 大豆愈伤组织序参量逐渐减小, 大豆愈伤组织序参量的变化很好地反映了UV-B辐射对组织细胞的伤害。

In order to evaluate the method of UV-B radiation damage on plant cell by photonic technology, soybean callus was radiated by 50 μW/cm2 UV-B for 16 h. Ultraweak photon emission from soybean callus was collected and analyzed. The results showed that strength of spontaneous luminescence (SL) in ultraweak photon emission of soybean callus increased and delayed luminescence (DL) decreased gradually. Through the analyses of dynamic presses of DL, the change rules of dynamic parameters of DL such as integrated inten-sity I(T), initial I0, correlation time τ and decay constant β were obtained. It was indicated that integrated intensity I(T), initial photon number I0, correlation time τ and decay constant β decreased with the UV-B radia-tion time, and I(T) was positive correlation to initial photon number I0. The order parameter used to describe order degree of cell was defined according to biological signification of ultraweak photon emission. The study found that the changes of the order parameter of soybean callus under the action of UV-B radiation reflected the damage of UV-B radiation on cell. So, the damage of UV-B radiation on plant cell could be expressed by the order parameter based on ultraweak photon emission of cell.