激光与光电子学进展, 2015, 52 (8): 081703, 网络出版: 2015-07-29
635nm/808nm双波长低能量激光对人成纤维细胞氧化应激反应的研究 
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Research of Oxidative Stress Reaction Induced by 635nm/808nm Double Wavelength Low Level Laser Therapy on CCC-ESF
医用光学 低能量激光 成纤维细胞 活性氧 生长因子 medical optics low level laser fibroblast reactive oxygen species growth factor
摘要
研究和比较双波长低能量激光疗法(LLLT)对人皮肤成纤维细胞(CCC-ESF)的生长、酶活性以及生长因子等的影响,讨论弱激光促进伤口愈合的作用机理.体外培养CCC-ESF,实验分为对照组和照光组(635 nm 组、808 nm 组和635 nm/808 nm 组),照光剂量为20 mW/cm2、12 J/cm2,分别检测细胞增殖情况、活性氧(ROS)产量、抗氧化酶活性[过氧化氢酶(CAT)和超氧化物歧化酶(SOD)]、脂质氧化能力(MDA)以及细胞生长因子含量.635 nm 组细胞增殖速度明显大于其他各组；635 nm、808 nm 和635 nm/808 nm 组ROS 水平分别比对照组提高了14.55%、7.49%和14.92%；CAT 和SOD 酶活力提高；808 nm 激光促进MDA 增加；双波长激光使人白介素1(IL-1)和成纤维细胞生长因子(FGF)分泌量显著增多；808 nm 激光增加了人胰岛素样生长因子(IGF-1)和人胶质细胞源性神经营养因子(GDNF)的分泌.LLLT 诱导产生的低水平氧化应激反应可促进CCC-ESF 增殖,提升细胞活力,增强细胞对外界环境的抵抗能力.LLLT 刺激细胞分泌多种细胞因子,调控创伤愈合的进程.双波长激光产生的生物效应优于单一波长.
Abstract
To investigate and compare the biological effect of proliferation,enzyme activity and growth factor after double wavelength low level laser therapy (LLLT) on human embryonic skin fibroblasts (CCC-ESF) in vito,and explore the molecular mechanism of wound healing,in vitro culture of CCC-ESF is divided into control group and experimental groups (635 nm group,808 nm group,635 nm/808 nm group),laser of 20 mW/cm2,12 J/cm2 is applied to detect cell proliferation,reactive oxygen species (ROS) production,antioxidant enzyme activity [catalase (CAT)and superoxide dismutase (SOD)],lipid oxidation (MDA) and amount of cytokines related.Cell proliferation rate in the 635 nm group is significantly higher than that in other groups;ROS levels are 14.55%,7.49% and 14.92% respectively in 635 nm,808 nm and 635 nm/808 nm groups,which are higher than that in the control group;CAT and SOD enzyme activity increases after LLLT;808 nm laser promotes the level of MDA and expression of interleukine-1 (IL-1) and fibroblast growth factor (FGF);808 nm laser can stimulate the secretion of insulin-like growth factor 1 (IGF-1) and glial cell line-derived neurotrophic factor (GDNF).Low levels of oxidative stress induced by LLLT can promote the proliferation of CCC-ESF,improve cell viability,and enhance cellular resistance to the external environment.LLLT stimulates the secretion of a variety of cytokines and regulates the wound healing process.Biological effect produced by double wavelength LLLT is better than that with a single wavelength in CCC-ESF.
陈洪丽, 王宏, 王超, 阴慧娟, 金文东, 穆志明, 李迎新. 635nm/808nm双波长低能量激光对人成纤维细胞氧化应激反应的研究[J]. 激光与光电子学进展, 2015, 52(8): 081703. Chen Hongli, Wang Hong, Wang Chao, Yin Huijuan, Jin Wendong, Mu Zhiming, Li Yingxin. Research of Oxidative Stress Reaction Induced by 635nm/808nm Double Wavelength Low Level Laser Therapy on CCC-ESF[J]. Laser & Optoelectronics Progress, 2015, 52(8): 081703.
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