激光生物学报, 2015, 24 (5): 460, 网络出版: 2015-11-30
金鱼Dishevelled3基因的克隆与分化表达研究
A Study on Cloning and Differential Expression of Gene Dishevelled 3 in Goldfish
散乱蛋白 Wnt信号转导通路 dishevelled dvl-3 dvl-3 wnt signal transduction pathway RT-PCR RT-PCR Western blot western blot
摘要
目的:本文主要以金鱼中的dvl-3基因为研究对象,研究dvl-3在金鱼不同的组织和胚胎发育时期中可能行使的功能。方法:采用RACE克隆技术获得金鱼dvl-3基因的cDNA全长,再利用半定量RT-PCR技术和蛋白免疫印记(Western blot)技术分析研究dvl-3基因在mRNA水平和蛋白水平上的表达差异。结果:在金鱼不同组织与胚胎发育时期,dvl-3基因在mRNA水平和蛋白水平上具有显著的表达差异性。结论:由此可见散乱蛋白dvl-3在金鱼不同的组织和胚胎发育时期中可能行使不同的功能,但其具体的功能及机制还有待进一步研究。
Abstract
Objective:Here,our study focused on gene dvl-3 in Carassius auratus. Research the function of dvl-3 proteins during embryonic development stages in Carassius auratus as well as in different tissues. Method: Firstly,we used RACE (rapid-amplification of cDNA ends) clone technology to obtain full-length cDNA of gene dvl-3,and then semi-quantitative RT-PCR and western blot were used to analyze the differential expression of dvl-3 in mRNA level and protein level respectively. Results:The results showed that: Semi-quantitative RT-PCR and semi-quantitative western blot results are different in different embryonic development and different tissues. Conclusions:Thus it showed that the function of dvl-3 proteins varied during embryonic development stages in Carassius auratus as well as in different tissues. However,the exact mechanism remained to be further explored.
邓美霞, 张卉琴, 张翠媛, 李林芳, 钟彬彬, 陈思, 周柳, 肖亚梅, 李万程, 刘文彬. 金鱼Dishevelled3基因的克隆与分化表达研究[J]. 激光生物学报, 2015, 24(5): 460. DENG Meixia, ZHANG Huiqin, ZHANG Cuiyuan, LI Linfang, ZHONG Binbin, CHEN Si, ZHOU Liu, XIAO Yamei, LI Wancheng, LIU Wenbin. A Study on Cloning and Differential Expression of Gene Dishevelled 3 in Goldfish[J]. Acta Laser Biology Sinica, 2015, 24(5): 460.