研究了具有生物相容性的低聚壳聚糖衍生物与活性药物牛血清白蛋白(BSA)之间的作用。 合成了聚合度为20的窄分子量分布低聚壳聚糖 (NLCS20)的3种衍生物: N-马来酰化低聚壳聚糖(MNLCS)、 N-羧甲基低聚壳聚糖(CNLCS)和N-琥珀酰化低聚壳聚糖(SNLCS)。 通过紫外光谱(UV)、 荧光光谱和等温滴定微量热仪(ITC)研究了NLCS20衍生物与BSA之间的相互作用。 结果表明, BSA的构象在NLCS20衍生物溶液微环境中没有发生变化, 活性也没有受到影响。 NLCS20衍生物与BSA的作用过程是放热的且是自发进行的, 主要作用力是氢键和疏水作用。 说明低聚壳聚糖衍生物能够作为潜在的亲水药物如活性医药蛋白或氨基酸的运输载体。

The effect between biocompatible low molecular weight chitosan derivatives as the delivery carrier of active pharmaceuticals bovine serum album (BSA) was studied. Three derivatives of low molecular weight chitosan with 20 degree of polymerization (NLCS20) and narrow molecular weight distribution including N-maleoyl-NLCS20, N-carboxymethyl-NLCS20 and N-succinyl-NLCS20 were synthesized. The effect between NLCS20 derivatives and BSA was studied with UV spectrum, fluorescence spectrum and isothermal titration calorimetry (ITC). The results showed that the conformation and activity of BSA did not change a lot in the microenvironment of NLCS20 derivatives solution. The process was exothermic and spontaneous. The main binding force was hydrogen bond and hydrophobic interaction. It was concluded that low molecular weight derivatives are likely to be delivery carriers of hydrophilic drugs such as active protein or poly amino acid.