Wide-field <i>in situ</i> multiplexed Raman imaging with superresolution

Houkai Chen; Xiaojing Wu; Yuquan Zhang; Yong Yang; Changjun Min; Siwei Zhu; Xiaocong Yuan; Qiaoliang Bao; Jing Bu

doi:doi:10.1364/PRJ.6.000530

Photonics Research, 2018, 6 (6): 06000530, Published Online: Jul. 2, 2018

Wide-field in situ multiplexed Raman imaging with superresolution

Houkai Chen ¹Xiaojing Wu ²Yuquan Zhang ^1,*Yong Yang ³Changjun Min ¹Siwei Zhu ²Xiaocong Yuan ¹Qiaoliang Bao ⁴Jing Bu ^4,5

Author Affiliations

¹ Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Shenzhen University, Shenzhen 518060, China

² Tianjin Union Medical Center, Tianjin 300121, China

³ Institute of Modern Optics, Nankai University, Tianjin 300071, China

⁴ College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060, China

⁵ e-mail: jingbu@szu.edu.cn

Figures & Tables

Fig. 1. Schematic of wide-field superresolution Raman imaging system.

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Fig. 2. Typical Raman spectra of the three different encoded SERS nanoparticles. Asterisks (*) indicate the selected peaks, and colored rectangular areas indicate the spectral regions that were used for multiplexed imaging in the experiments.

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Fig. 3. (a) Normalized intensities of fluorescent scattering and Raman scattering signals over time. The signals were detected at a frame rate of 0.2 Hz over an exposure time of 0.3 s. The red curve represents the Raman signal, and the black curve represents the fluorescent signal. (b) and (c) show time-lapse images of the fluorescent microspheres and the SERS nanoparticles, respectively.

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Fig. 4. Wide-field imaging of the SERS nanoparticles. (a) Conventional imaging results and (b) the reconstructed wide-field image; (c) Gaussian fitting profiles of the normalized intensity of the images framed in (a) and (b). Scale bar: 1 μm.

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Fig. 5. Multiplexed imaging of 3T3 cells labeled using surface-modified SERS nanoparticles. (a) Bright field image of 3T3 cell, and (b) conventional and (c) reconstructed wide-field pseudo-color images overlaid with the framed bright field image from (a). Scale bars: 5 μm in (a), and 1 μm in (b) and (c).

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Houkai Chen, Xiaojing Wu, Yuquan Zhang, Yong Yang, Changjun Min, Siwei Zhu, Xiaocong Yuan, Qiaoliang Bao, Jing Bu. Wide-field in situ multiplexed Raman imaging with superresolution[J]. Photonics Research, 2018, 6(6): 06000530.

Wide-field in situ multiplexed Raman imaging with superresolution

Fig. 1. Schematic of wide-field superresolution Raman imaging system.

Fig. 2. Typical Raman spectra of the three different encoded SERS nanoparticles. Asterisks (*) indicate the selected peaks, and colored rectangular areas indicate the spectral regions that were used for multiplexed imaging in the experiments.

Fig. 4. Wide-field imaging of the SERS nanoparticles. (a) Conventional imaging results and (b) the reconstructed wide-field image; (c) Gaussian fitting profiles of the normalized intensity of the images framed in (a) and (b). Scale bar: 1 μm.

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Wide-field in situ multiplexed Raman imaging with superresolution

Fig. 1. Schematic of wide-field superresolution Raman imaging system.

Fig. 2. Typical Raman spectra of the three different encoded SERS nanoparticles. Asterisks (*) indicate the selected peaks, and colored rectangular areas indicate the spectral regions that were used for multiplexed imaging in the experiments.

Fig. 4. Wide-field imaging of the SERS nanoparticles. (a) Conventional imaging results and (b) the reconstructed wide-field image; (c) Gaussian fitting profiles of the normalized intensity of the images framed in (a) and (b). Scale bar: 1 μm.

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