发光学报, 2018, 39 (10): 1371, 网络出版: 2018-11-25   

近红外到近红外Mn2+掺杂NaYF4 ∶Yb3+/Tm3+纳米粒子的制备及其生物成像

Synthesis of NIR-to-NIR Mn2+ Doped- NaYF4 ∶Yb3+/Tm3+ Nanoparticles and Their Applications in Bioimaging
作者单位
西南民族大学 化学与环境保护工程学院, 四川 成都 610041
摘要
在反应温度为200 ℃、反应时间为8 h的温和条件下, 采用水热法合成了近红外到近红外的Mn2+掺杂NaYF4 ∶Yb3+/Tm3+上转换荧光纳米粒子, 再以两亲性聚合物C18PMH-mPEG作为亲水性配体修饰到上转换荧光纳米粒子表面, 得到具有水溶性的上转换荧光纳米粒子。然后在980 nm近红外光源激发下, 测量了上转换荧光纳米粒子的荧光发射光谱, 在(800±10) nm附近, 观察到了较强的单近红外光发射(3H4 →3H6)。对样品进行细胞毒性实验, 结果表明制得的水溶性Mn2+掺杂NaYF4 ∶Yb3+/Tm3+纳米粒子具有良好的生物相容性。并进一步在小鼠体内进行了近红外成像, 表明其在生物成像领域将会具有一定的应用前景。
Abstract
Mn2+doped- NaYF4 ∶Yb3+/Tm3+ upconversion fluorescence nanoparticles(NPs) were synthesized by one-step hydrothermal method under 200 ℃ for 8 h, and then the hydrophobic nanoparticles were transferred into water through coating amphiphilic polymer C18PMH-mPEG. Upconversion fluorescence emission spectrum of Mn2+doped- NaYF4 ∶Yb3+/Tm3+ NPs was measured under excitation by a near-infrared(NIR) light at 980 nm. The results of cytotoxicity test show that the water-soluble Mn2+doped- NaYF4 ∶Yb3+/Tm3+ NPs have good biocompatibility. Moreover, the prepared Mn2+doped- NaYF4 ∶Yb3+/Tm3+ NPs are used for in mice NIR imaging, which shows that it will have a certain application prospect in bioimaging.
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罗阳, 廖正芳, 张伟, 左芳. 近红外到近红外Mn2+掺杂NaYF4 ∶Yb3+/Tm3+纳米粒子的制备及其生物成像[J]. 发光学报, 2018, 39(10): 1371. LUO Yang, LIAO Zheng-fang, ZHANG Wei, ZUO Fang. Synthesis of NIR-to-NIR Mn2+ Doped- NaYF4 ∶Yb3+/Tm3+ Nanoparticles and Their Applications in Bioimaging[J]. Chinese Journal of Luminescence, 2018, 39(10): 1371.

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