光学学报, 2010, 30 (12): 3563, 网络出版: 2010-12-07   

显微干涉术在血红细胞光相位场定量测量中的应用

Application of Microscopic Interferometry for Quantitative Phase Measurement of Red Blood Cells
作者单位
南京理工大学理学院, 江苏 南京 210094
摘要
为提高生物细胞形态检测速度和精度,以典型相位物体血红细胞为研究对象,结合离轴干涉、共焦显微和高速图像采集技术,提出了细胞光相位场定量测量方法,既有高测量精度又可以对流动的细胞进行实时放大观测。实验中采集单幅载频干涉图,运用快速傅里叶变换相位提取法和路径无关相位解包算法,最终获得血红细胞光相位场的定量分布,和已有的光学相位模型吻合得较好。分析表明,该系统分辨率达到亚微米量级。这种由细胞光相位场分布解构细胞形态的检测手段希望成为一种新的生物活体细胞实时在体检测和定量分析的方法。
Abstract
In order to improve detection speed and accuracy of biological cells, microscopic interferometry which is a noncontact technique for imaging of phase objects is proposed, i.e., red blood cells. The technique combines the principles of off-axis interferometry, confocal microscopy and high-speed image-capture technology and is characterized by optimized spatial resolution and real-time acquisition capabilities. Single carrier-frequency interferogram is captured from experimental setup. According to fast Fourier transform phase retrieval method and path-unfollowing unwrapping algorithm, the quantitative phase distributions of red blood cell are gained and agree well with the previous optical phase models. Analysis shows that the resolution of introduced system reaches sub-micron dimension. It provides a breakthrough method for real-time observing and quantitative analyzing of cells in vivo.
参考文献

[1] . Huang, E. A. Swanson, C. P. Lin et al.. Optical coherence tomography[J]. Science, 1991, 254(5035): 1178-1181.

[2] 李鹏, 黄润, 高万荣. 光学相干层析术在人体皮肤成像方面的实验研究[J]. 中国激光, 2009, 36(10): 2498~2502

    Li Peng, Huang Run, Gao Wanrong. Experiment research on optical coherence tomography of human skin[J]. Chinese J. Lasers, 2009, 36(10): 2498~2502

[3] 段炼, 何永红, 朱锐 等. 三维谱域光学相干层析成像系统的研制[J]. 中国激光, 2009, 36(10): 2528~2533

    Duan Lian, He Yonghong, Zhu Rui et al.. Development of a spectrum domain 3D optical coherence tomography system[J]. Chinese J. Lasers, 2009, 36(10): 2528~2533

[4] 王亚伟, 雷海娜,卜敏 等. 几种典型血细胞的光学相位模型及其分布特征与识别方法[J]. 中国激光, 2009, 36(10): 2629~2635

    Wang Yawei, Lei Haina, Bu Min et al.. Distribution characteristics and identification of several typical blood cells under optical phase models[J]. Chinese J. Lasers, 2009, 36(10): 2629~2635

[5] 王亚伟, 韩广才, 刘莹 等. 双曲面对称体红细胞模型的光散射虚拟仿真[J]. 中国激光, 2007, 34(12): 1676~1681

    Wang Yawei, Han Guangcai, Liu Yin et al.. Light scattering virtual simulation of red blood cell under double curve symmetrical model[J]. Chinese J. Lasers, 2007, 34(12): 1676~1681

[6] . J. Stephens, V. J. Allan. Light microscopy techniques for live cell imaging[J]. Science, 2003, 300(5616): 82-86.

[7] . Popescu, L. P. Deflores, J. C. Vaughan et al.. Fourier phase microscopy for investigation of biological structures and dynamics[J]. Opt. Lett., 2004, 29(21): 2503-2505.

[8] . Ding, Z. Wang, F. Nguyen et al.. Fourier transform light scattering of inhomogeneous and dynamic structures[J]. Phys. Rev. Lett., 2008, 101(23): 238102.

[9] . Ikeda, G. Popescu, R. R. Dasari et al.. Hilbert phase microscopy for investigating fast dynamics in transparent systems[J]. Opt. Lett., 2005, 30(10): 1165-1167.

[10] . K. Park, G. Popescu, K. Badizadegan et al.. Diffraction phase and fluorescence microscopy[J]. Opt. Express, 2006, 14(18): 8263-8268.

[11] 王亚伟, 韩广才, 刘莹 等. 血液细胞光相位分布特征的虚拟仿真研究[J]. 中国激光, 2009, 36(6): 1595~1600

    Wang Yawei, Han Guangcai, Liu Ying et al.. Simulation study on the phase distribution of blood cells by virtual imitation[J]. Chinese J. Lasers, 2009, 36(6): 1595~1600

[12] 袁润, 王亚伟, 蔡兰. 红细胞的几种光学近似模型及其光信息分布特征[J]. 中国激光, 2009, 36(10): 2614~2618

    Yuan Run, Wang Yawei, Cai Lan. Several optical approximate model and light message distribution characteristics of red blood cells[J]. Chinese J. Lasers, 2009, 36(10): 2614~2618

[13] . Choi, C. Fang-Yen, K. Badizadegan et al.. Tomographic phase microscopy[J]. Nat. Methods, 2007, 4(9): 717-719.

[14] G. N. Vishnyakov, G. G. Levin. Interferometric computed-microtomography of 3D phase objects[C]. SPIE, 1997, 2984: 64~71

[15] Xue Liang, Lai Jiancheng, Li Zhenhua. Determining the optimal imaging position in tomographic interference microscopy[C]. SPIE, 2009, 7513: 75130Z-1

[16] . Iwai, C. Fang-Yen, G. Popescu. Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry[J]. Opt. Lett., 2005, 29(20): 2399-2401.

[17] 王超, 冯国英. 从一幅载频全息图中实现相位重构的新算法[J]. 光学学报, 2008, 28(7): 1269~1273

    Wang Chao, Feng Guoying. A new algorithm for phase reconstruction from a single carrier-frequency interferogram[J]. Acta Optica Sinica, 2008, 28(7): 1269~1273

[18] 陈秀丽, 王桂文, 尹晓林 等. 单细胞拉曼光谱分析地中海贫血红细胞的氧合态和去氧态[J]. 光学学报, 2009, 29(10): 2854~2859

    Chen Xiuli, Wang Guiwen, Yin Xiaolin et al.. Single-cell Raman spectral analysis of oxygenated and deoxygenated thalassemia erythrocyte[J]. Acta Optica Sinica, 2009, 29(10): 2854~2859

[19] 董可平, 钱晓凡, 张磊 等. 数字全息显微术对细胞的研究[J]. 光子学报, 2007, 36(11): 2013~2016

    Dong Keping, Qian Xiaofan, Zhang Lei et al.. Digital holographic microscopy study for cells[J]. Acta Photonica Sinica, 2007, 36(11): 2013~2016

薛亮, 来建成, 王绶玙, 李振华. 显微干涉术在血红细胞光相位场定量测量中的应用[J]. 光学学报, 2010, 30(12): 3563. Xue Liang, Lai Jiancheng, Wang Shouyu, Li Zhenhua. Application of Microscopic Interferometry for Quantitative Phase Measurement of Red Blood Cells[J]. Acta Optica Sinica, 2010, 30(12): 3563.

本文已被 2 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!