全场高分辨生物组织光学层析成像

朱越; 高万荣

doi:doi:10.3788/cjl201441.0804002

中国激光, 2014, 41 (8): 0804002, 网络出版: 2014-07-15

全场高分辨生物组织光学层析成像下载： 650次

High-Resolution Full-Field Optical Coherence Tomography for Biological Tissue

论文大纲

朱越 ^*高万荣

作者单位

南京理工大学电光学院光学工程系, 江苏南京 210094

成像系统全场光学相干层析术移相干涉术生物组织 imaging systems full-field optical coherence tomography phase-shifting interferometry biological tissue

摘要

建立了一套全场光学相干层析(FFOCT)系统，以实现对生物组织和细胞的高分辨层析成像。该光学系统基于Linnik干涉显微结构。不同于现有FFOCT系统采用光纤束照明方式，采用卤钨灯照明和大数值孔径显微物镜成像，压电陶瓷(PZT)移相，面阵电荷耦合器件(CCD)采集干涉信号，由5步移相算法获取层析图，最终合成三维图像。对整套系统的性能进行了详细的阐述与分析，并通过对洋葱表皮细胞和盆栽树叶的光学层析实验,验证了本系统的可行性和精确度。通过对集成电路内部芯片（英特尔奔腾4，横向分辨率达0.8 μm）成像，证明了该系统的分辨率可达到0.7 μm×0.5 μm（横向×纵向）。提出的系统分辨率高、成本低、结构简单便于调节，为实现高分辨率光学相干层析成像提供了简单易行的方法。

Abstract

A novel full-field optical coherence tomography (FFOCT) system with low cost and high resolution is developed for imaging of cells and tissues. Different from other FFOCT systems illuminated with optical fiber bundle, the improved Khler illumination arrangement with a halogen lamp is used in the proposed FFOCT system. High numerical aperture microscopic objectives are used for imaging and a piezoelectric ceramic transducer (PZT) is used for phase-shifting. En-face tomographic images can be obtained by applying the five-step phase-shifting algorithm to a series of interferometric images which are recorded by a smart charge coupled device (CCD) camera. Three-dimensional images can be generated from these tomographic images. Imaging of the chip of Intel Pentium 4 processor demonstrats the ultrahigh resolution of the system (lateral resolution 0.8 μm), approaching the theoretical resolution 0.7 μm×0.5 μm (lateral×axial). En-face images of cells of onion surface and potted plant leaves cells show the excellent performance of the system for generating en-face images of biological tissues. The system is characterized by its high resolution, low cost and simple arrangement for adjustment, providing a practical method of performing FFOCT imaging.

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朱越, 高万荣. 全场高分辨生物组织光学层析成像[J]. 中国激光, 2014, 41(8): 0804002. Zhu Yue, Gao Wanrong. High-Resolution Full-Field Optical Coherence Tomography for Biological Tissue[J]. Chinese Journal of Lasers, 2014, 41(8): 0804002.

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