基于2D-COS红外光谱的附子炮制过程时序段解析研究

采用傅里叶变换红外光谱技术, 获得蒸制过程中附子红外光谱及二维相关谱（Tow-dimensional correlation, 2D-COS）, 研究附子炮制过程特征。分别比较原始红外光谱、二阶导数谱及二维相关谱, 结果表明红外原始光谱图十分相似, 结合二阶导数光谱分析1　63487 cm-1为羰基峰; 1　60318, 1　57158, 1　48509和1　41308 cm-1为苯环的骨架振动峰, 85141和75924 cm-1 为苯环C—H键的振动峰; 1　15373, 　1　08184和1　02135 cm-1为生物碱中酯键和醇羟基的特征峰。二维相关谱结果揭示附子在炮制过程中发生的2个过程时序段, 分别为2～3和8～9　h。研究数据有助于临床根据适应证选择适宜的煎煮时间, 避免煎煮不足导致中毒或煎煮太过影响疗效。

Abstract

Fourier transform infrared spectroscopy (FTIR), second derivative infrared spectroscopy, and two-dimensional correlation infrared spectroscopy (2D-IR) were used to study the steaming procedure of aconite tuber. The results showed that samples with steaming procedures were similar to each other in one-dimensional IR spectra but different in 2D-IR spectra. The FTIR spectra combined with second derivative infrared spectroscopy of different samples during boiling procedure present characteristic peaks at 1　63487 cm-1 (CO), 1　60318, 1　57158 cm-1, 1　48509, 1　41308 cm-1 (vibration of phenyl framework) and 1　15373, 1　08184, 1　02135 cm-1 (C—O) respectively. There were two conspicuous time sequences in two dimensional infrared correlation spectroscopy of autopeaks intensity. Since, the intensity of autopeaks was relatively high during 2～3 and 8～9　h. The function of FTIR and 2D-IR for analysis of the alkaloid from aconite root attributed high speed and high efficiency. The results were reliable and valuable.

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吴志生, 刘晓娜, 谭鹏, 裴艳玲, 戴幸星, 乔延江. 基于2D-COS红外光谱的附子炮制过程时序段解析研究[J]. 光谱学与光谱分析, 2017, 37(6): 1745. WU Zhi-sheng, LIU Xiao-na, TAN Peng, PEI Yan-ling, DAI Xing-xing, QIAO Yan-jiang. Research on Steaming Procedure of Aconite Root with FTIR and Two-Dimensional IR Correlation Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2017, 37(6): 1745.

基于2D-COS红外光谱的附子炮制过程时序段解析研究

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