拟威布尔分布密度函数在荧光寿命成像数据分析中的应用

周明华; 隋成华

光学学报, 2005, 25 (6): 835, 网络出版: 2006-05-22

拟威布尔分布密度函数在荧光寿命成像数据分析中的应用

Application of the Quasi-Weibull Distribution Density Function to Data Analysis of Fluorescence Lifetime Imaging

论文大纲

周明华 ^1,*隋成华 ²

作者单位

¹ 浙江工业大学应用数学系, 杭州 310032

² 浙江工业大学应用物理系, 杭州 310032

生物光学拟威布尔分布密度函数荧光寿命法成像技术荧光衰减过程 biological optics quasi-Weibull distribution density function fluorescence lifetime imaging fluorescence decay profile

摘要

荧光寿命法成像技术(FLIM)是一种非常有效、功能强大且能用来分析复杂生物组织和细胞分子的成像技术。传统的荧光寿命成像的数据分析,按某些具有不同寿命、离散的单参量指数模型来描述荧光衰减过程。在生物组织这样既复杂又不均匀的样品中,虽然多参量指数模型能提供比单参量指数模型对实验数据更好的拟合效果,但是离散多参量的假定往往是随意的。提出了拟威布尔分布密度函数可能是生物荧光分子团衰减动力过程的真实再现,并且通过计算证明,对于某些生化感兴趣的荧光分子团的多槽基面效价测定样品的数据,相对于单参量指数与多参量指数衰减函数有更好的一致性。同时讨论了将该荧光衰减模型应用于荧光寿命成像的前景。

Abstract

Fluorescence lifetime imaging is a rather effective and powerful method that can be used to analyze complex biological tissues and molecules. Conventional analyses of fluorescence lifetime data resolve the fluorescence decay profile in terms of discrete single exponential components with distinct lifetimes. In complex, heterogeneous biological samples such as tissue, multi-exponential decay functions can appear to provide a better fit to fluorescence decay data than the assumption of a single-exponential decay, but the assumption of multiple discrete components is essentially arbitrary. Quasi-Weibull distribution density function was likely to provide a truer representation of the underlying fluorescence dynamics. As comparing with those of single exponential and multi-exponential decay functions, the novel model can yield the better goodness of fit using data from multi-well plate assays of chemically and biologically interesting fluorophores. At the same time, the potential application of the quasi-Weibull distribution density function to fluorescence lifetime imaging was discussed.

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周明华, 隋成华. 拟威布尔分布密度函数在荧光寿命成像数据分析中的应用[J]. 光学学报, 2005, 25(6): 835. 周明华, 隋成华. Application of the Quasi-Weibull Distribution Density Function to Data Analysis of Fluorescence Lifetime Imaging[J]. Acta Optica Sinica, 2005, 25(6): 835.

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