中国激光, 1998, 25 (8): 759, 网络出版: 2006-10-18  

氦氖激光诱导离体小鼠胸腺细胞凋亡的实验研究

Research of Apoptotic Mice Thymocytes in Vitro Generated by He-Ne Laser Irradiation
作者单位
上海第二医科大学生物物理教研室,上海 200025
摘要
实验发现随着低功率氦氖激光照射时间的延长(激光剂量的增加),胸腺细胞凋亡的相对数相应增大,表现在其DNA直方图上可见亚二倍体峰升高。
Abstract
An instant elevation in intracellular Ca 2+ concentration ([Ca 2+] i) appears significant after the irradiation of cells with a He-Ne laser. Studies using various cell types have demonstrated changes in DNA fluorescence that were attributable to a process of the apoptosis. The apoptosis is activation of an endonuclease, which has relation to the intracellulle [Ca 2+] i. The study has discovered an increase of the apoptosis of the mice thymocytes following the irradiation time elongation with the He-Ne laser. The apoptosis shows an elevation of the subdiploid peak in a histogram.
参考文献

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刘炳荣, 史桂英, 徐清. 氦氖激光诱导离体小鼠胸腺细胞凋亡的实验研究[J]. 中国激光, 1998, 25(8): 759. 刘炳荣, 史桂英, 徐清. Research of Apoptotic Mice Thymocytes in Vitro Generated by He-Ne Laser Irradiation[J]. Chinese Journal of Lasers, 1998, 25(8): 759.

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