中国激光, 2020, 47 (2): 0207039, 网络出版: 2020-02-21
基于光学相干层析成像散射量化表征细胞分布的研究 下载: 1197次
Characterization of Cell Distribution Based on Optical Coherence Tomography Scattering
图 & 表
图 1. 散射系数分布图像重建过程
Fig. 1. Reconstruction process of scattering coefficient distribution image
图 2. 多层介质散射模型的OCT图像及散射系数分布。(a) OCT强度图像;(b)本文方法计算的散射系数分布图像;(c)图2 (b)中白色虚线框处模型对应的散射曲线
Fig. 2. OCT image and scattering coefficient distribution of the multilayer dielectric scattering model. (a) OCT intensity image; (b) scattering coefficient distribution calculated by the proposed method; (c) scattering curve corresponding to the model at the white dotted frame in Fig.2 (b)
图 3. 不同浓度的C3A细胞悬浮液OCT强度图像与散射图像。(a1)~(a4) OCT强度图像;(b1)~(b4) OCT散射图像
Fig. 3. OCT intensity images and scattering images of C3A cell suspension with different concentrations. (a1)-(a4) OCT intensity images; (b1)-(b4) OCT scattering images
图 4. 不同浓度细胞悬液的OCT强度及散射系数的频数分布直方图。(a1)~(a4)信号强度;(b1)~(b4)散射系数
Fig. 4. Frequency distribution histograms of OCT intensity and scattering coefficient for cell suspension with different concentrations. (a1)-(a4) Signal intensity; (b1)-(b4) scattering coefficient
图 5. 分布直方图最大频数对应的参数值与细胞浓度的关系曲线。(a)强度值与细胞浓度的关系曲线;(b)散射系数值及背景峰和细胞特征峰的间距与细胞浓度的关系曲线
Fig. 5. Relationship between the parameters of the maximum frequency of the distribution histograms and the cell concentration. (a) Relationship between intensity and cell concentration; (b) relationship among scattering coefficient, distance between background peak and cell characteristic peak, and cell concentration
图 6. 无细胞和含细胞水凝胶支架的OCT强度图像以及散射图像。(a1)无细胞的支架的OCT强度图像;(a2)含细胞的支架的OCT强度图像;(b1)无细胞的支架的散射图像;(b2)含细胞的支架的散射图像
Fig. 6. OCT intensity images and scattering images of cell-free and cell-containing hydrogel scaffolds. (a1) OCT intensity image of cell-free scaffold; (a2) OCT intensity image of cell-containing scaffold; (b1) scattering image of cell-free scaffold; (b2) scattering image of cell-containing scaffold
图 7. 水凝胶支架的信号强度与散射系数分布直方图。(a) PBS缓冲溶液的强度分布;(b)无细胞的支架的强度分布;(c)含细胞的支架的强度分布;(d) PBS缓冲溶液的散射系数分布;(e)无细胞的支架的散射系数分布;(f)含细胞的支架的散射系数分布
Fig. 7. Histogram of signal intensity and scattering coefficient of hydrogel scaffold. (a) Intensity distribution of the PBS buffer solution; (b) intensity distribution of cell-free scaffold; (c) intensity distribution of cell-containing scaffold; (d) scattering corefficient distribution of PBS buffer solution; (e) scattering corefficient distribution of cell-free scaffold; (f) Scattering corefficient distribution cell-containing scaffold
图 8. 水凝胶支架的OCT强度图像、散射图像与H&E染色切片结果对比。(a1)无细胞水凝胶支架的OCT强度图像;(a2)细胞负载水凝胶支架的OCT强度图像;(b1)无细胞水凝胶支架的散射图像;(b2)细胞负载水凝胶支架的散射图像;(c1)无细胞水凝胶支架染色切片图;(c2)细胞负载水凝胶支架染色切片图
Fig. 8. Comparison of OCT intensity images, scattering images and H&E stained sections for hydrogel scaffolds. (a1) OCT intensity image of cell-free hydrogel scaffold; (a2) OCT intensity image of cell-containing hydrogel scaffold; (b1) scattering image of cell-free hydrogel scaffold; (b2) scattering image of cell-containing hydrogel scaffold; (c1) stained sections image of cell-free hydrogel scaffold; (c2) stained sections image of cell-containing hydrogel scaffold
表 1分布直方图频数峰值处的强度和散射系数及其标准差
Table1. Intensity and scattering coefficient, and their standard deviation at the peak of frequency of histogram
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沈仁强, 王玲, 徐铭恩, 彭世昌. 基于光学相干层析成像散射量化表征细胞分布的研究[J]. 中国激光, 2020, 47(2): 0207039. Shen Renqiang, Wang Ling, Xu Ming''en, Peng Shichang. Characterization of Cell Distribution Based on Optical Coherence Tomography Scattering[J]. Chinese Journal of Lasers, 2020, 47(2): 0207039.