光学学报, 2007, 27 (1): 129, 网络出版: 2007-01-22   

不同产地黑木耳的傅里叶变换红外光谱鉴别

Identification of Auricularia Auricula from Different Regions by Fourier Transform Infrared Spectroscopy
作者单位
1 云南师范大学物理系, 昆明 650092
2 云南师范大学分析测试中心, 昆明 650092
3 昆明食用菌研究所, 昆明 650223
摘要
利用傅里叶变换红外光谱法研究了五种不同产地的黑木耳。结果显示, 它们的红外光谱主要由蛋白质、脂类化合物及多糖的振动吸收谱带组成。五个样品的傅里叶变红外光谱整体上十分相似, 因此分别选取1750~1500 cm-1和1200~1000 cm-1范围内的傅里叶变换红外光谱进行相关性分析。结果显示,不同产地的黑木耳在1750~1500 cm-1范围内相关性较好, 光谱的相关系数分布在0.895~0.994。五个样品在1200~1000 cm-1范围内的相关系数分布在0.441~0.981, 东北地区产的黑木耳与西南地区产的黑木耳的光谱图有明显区别。利用1200~1000 cm-1范围内的差异并结合其它吸收峰的特征可鉴别出不同产地的黑木耳。研究结果表明傅里叶变换红外光谱在鉴别菌类方面具有方便、快速等优点。
Abstract
Fourier transform infrared spectroscopy (FTIR) was used to identify five kinds of Auricularia auricula from different regions. The results show the infrared spectrum of Auricularia auricula is mainly composed of the absorption of protein, aliphatic compound and polysaccharide. The spectra of five samples are very similar. Two regions of 1750~1500 cm-1 and 1200~1000 cm-1 in infrared absorption spectra were selected for evaluating the correlation coefficients. The correlation coefficients of five samples between 1750 cm-1 and 1500 cm-1 are located from 0.895 to 0.994, which indicates the spectra have good correlation. The correlation coefficients are located from 0.441 to 0.981 between 1200 cm-1 and 1000 cm-1. There are obvious differences about the spectra among the mushrooms in the northeast and southwest. The differences in the range 1200~1000 cm-1 and other characteristics of absorption peaks might be used to identify Auricularia auricula from different regions. It is proved that mushrooms can be identified rapidly and conveniently by Fourier transform infrared spectroscopy.
参考文献

[1] Huang Lainian. Colored Illustrations of Macrofungi (Mushrooms) of China[M]. Beijing: China Agricultural Press, 1998. 28~29 (in Chinese)
黄来年主编. 中国大型真菌原色图鉴[M]. 北京: 中国农业出版社, 1998. 28~29

[2] . Conformational change of the β-D-glucan of Auricularia auricula-judae in water-dimethyl sulfoxide mixtures[J]. Carbohydrate Research, 1995, 276(2): 443-447.

[3] Zhao Jiewen, Zhang Haidong, Liu Muhua. Preprocessing methods of near-infrared spectra for simplifying prediction model of sugar content of apples[J]. Acta Optica Sinica, 2006, 26(1): 136~140 (in Chinese)
赵杰文, 张海东, 刘木华. 简化苹果糖度预测模型的近红外光谱预处理方法[J]. 光学学报, 2006, 26(1): 136~140

[4] Wu Jinguang. Techniques and Applications of Modern Fourier Transform Infrared Spectroscopy[M]. Beijing: Science and Technology Literature Press, 1994. 156~180 (in Chinese)
吴瑾光主编. 近代傅里叶变换红外光谱技术及应用[M]. 北京: 科学技术文献出版社, 1994. 156~180

[5] Kan Ruifeng, Liu Wenqing, Zhang Yujun et al.. Infrared absorption spectrometer of monitoring ambient methane[J]. Acta Optica Sinica, 2006, 26(1): 67~70 (in Chinese)
阚瑞峰, 刘文清, 张玉钧 等. 基于可调谐激光吸收光谱的大气甲烷监测仪[J]. 光学学报, 2006, 26(1): 67~70

[6] Liu Gang, Liu Jianhong, Yang Aiming et al.. Identification of edible mushrooms by fourier transform infrared spectroscopy[J]. Spectroscopy and Spectral Analysis, 2004, 24(8): 941~945 (in Chinese)
刘刚, 刘剑虹, 杨爱明 等. 食用菌的傅里叶变换红外光谱鉴别[J]. 光谱学与光谱分析, 2004, 24(8): 941~945

[7] . L. S. Albuquerque, I. Guedes, P. Alcantara Jr. et al.. Infrared absorption spectra of Buriti (Mauritia flexuosa L.) oil[J]. Vibrational Spectroscopy, 2003, 33(1): 127-131.

[8] . Kamnev, Marinela Colina, Jose Rodriguez et al.. Comparative spectroscopic characterization of different pectins and their sources[J]. Food Hydrocolloids, 1998, 12(3): 263-271.

[9] Han Runping, Bao Gailing, Zhu Lu. Comparison of infrared spectra of native and esterified beer yeast[J]. Spectroscopy and Spectral Analysis, 2004, 24(7): 820~822 (in Chinese)
韩润平, 鲍改玲, 朱路. 酯化前后酵母菌的红外光谱比较[J]. 光谱学与光谱分析, 2004, 24(7): 820~822

[10] Liu Gang, Liu Jianhong, Song Dingshan et al.. Fourier transform infrared spectroscopic study of different parts of wild growing edible mushrooms[J]. Spectroscopy and Spectral Analysis, 2005, 25(7): 1053~1056
刘刚, 刘剑虹, 宋鼎珊 等. 野生食用蕈菌不同部位的红外光谱研究[J]. 光谱学与光谱分析, 2005, 25(7): 1053~1056

[11] . Puppels. Vibrational spectroscopic characterization of wild growing mushrooms and toadstools[J]. Spectrochimica Acta Part A, 2001, 57(14): 2815-2829.

[12] . Khalique Ahmed, James K.Daun, Roman Przybylski. FT-IR based methodology for quantitation of total tocopherols, tocotrienols and plastochromanol-8 in vegetable oils[J]. J. Food Composition and Analysis, 2005, 18(5): 359-364.

[13] Liu Gang, Xing Da, Wang Haimin et al.. Study of protein in human gallstones by Fourier transform infrared spectroscopy and surface-enhanced Raman spectroscopy[J]. Acta Optica Sinica, 2002, 22(4): 441~446 (in Chinese)
刘刚, 邢达, 王海珉 等. 胆结石中蛋白质的傅里叶变换红外光谱和表面增强拉曼光谱研究[J]. 光学学报, 2002, 22(4): 441~446

[14] . Smith, Michael J. Gidley et al.. Molecular interactions in bacterial cellulose composites studied by 1D FT-IR and dynamic 2D FT-IR spectroscopy[J]. Carbohydrate Research, 2002, 337(12): 1145-1153.

[15] . . Microbial (1→3)-β-D-glucans, their preparation, physico-chemical characterization and immunomodulatory activity[J]. Carbohydrate Polymers, 1999, 38(3): 247-253.

[16] . Carpita, Marianne Defernez, Kim Findlay et al.. Cell wall architecture of the elongating maize coleoptile[J]. Plant Physiology, 2001, 127(2): 551-565.

[17] . Haxaire, Y. Marechal, M. Milas et al.. Hydration of polysaccharide hyaluronan observed by IR spectrometry. I. preliminary experiments and band assignments[J]. Biopolymers, 2003, 72(1): 10-20.

时有明, 刘刚, 刘剑虹, 宋鼎珊. 不同产地黑木耳的傅里叶变换红外光谱鉴别[J]. 光学学报, 2007, 27(1): 129. 时有明, 刘刚, 刘剑虹, 宋鼎珊. Identification of Auricularia Auricula from Different Regions by Fourier Transform Infrared Spectroscopy[J]. Acta Optica Sinica, 2007, 27(1): 129.

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