Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

Tao Ma; Xiaoxia Chen; Qing Peng; Pengfei Zhang; Yonghong He

doi:doi:10.1142/s1793545816500401

Journal of Innovative Optical Health Sciences, 2017, 10 (2): 1650040, Published Online: Dec. 27, 2018

Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

论文大纲

Tao Ma ¹Xiaoxia Chen ²Qing Peng ^3,*Pengfei Zhang ^4,5Yonghong He ^4,5

Author Affiliations

¹ Department of Ophthalmology, Shanxi Eye Hospital, Taiyuan 030002, P. R. China

² Department of Ophthalmology, Xin Hua hospital affiliated to Shanghai, JiaoTong University School of Medicine, Shanghai 200092, P. R. China

³ Department of Ophthalmology, MinHang Hospital, Affiliated to Fudan University, Shanghai 201199, P. R. China

⁴ Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P. R. China

⁵ Department of Physics, Tsinghua University, Beijing 100084, P. R. China

Surface plasmon resonance surface functionalization dopamine molecular interac-tion analysis

Abstract

Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These opera-tions are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.

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Tao Ma, Xiaoxia Chen, Qing Peng, Pengfei Zhang, Yonghong He. Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition[J]. Journal of Innovative Optical Health Sciences, 2017, 10(2): 1650040.

Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

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