基于叶绿素荧光的浮游植物生长状态监测技术

浮游植物生长状态监测以叶绿素荧光为基础,利用叶绿素荧光技术可以获得有关光系统II的大量信息。以蓝藻和绿藻为主要研究对象,采用两个450 nm蓝光LED分别用于激发本底荧光Fo和使样本处于光适应状态,利用超高亮激光二级管作为饱和脉冲光源获得最大荧光Fm。利用自行搭建的系统测量正常光照培养和对照组放置于暗室浮游植物样品的光化学反应效率Yield,研究了不同光适应状态下的最大相对电子传递速率ETRmax、光能利用效率α和光损伤参数β,并记录了完整的荧光诱导曲线和光响应曲线,实现了浮游植物生长状态的测定。

Abstract

The monitoring of phytoplankton growth state was based on chlorophyll fluorescence, mainly by using the modulated fluorometer. The changes in PSII performance of the photosynthetic apparatus have been explored by applying chlorophyll fluorescence technique. Chlorophyll fluorescence of microcystis aeruginosa and chlorella vulgaris were measured by system developed in lab. 450 nm LED were selected as excitation light source, which was used to obtain fluorescence at pigment level and light-adaptation. LD was used to supply saturation pulse which could interrupt photochemical reaction briefly. The photochemical reaction efficiency Yield was measured between phytoplankton cultivated in normal light and in control which placed in a darkroom. The maximum relative electron transport rate ETRmax tested under different light-adapted state, light use efficiency α and light damage parameter β were investigated, fluorescence induction curves and light response curves were measured, and the determination of phytoplankton growth state can be achieved.

参考文献

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刘晶, 刘文清, 赵南京, 张玉钧, 马明俊, 戴庞达, 殷高方, 段静波, 余晓娅, 方丽, 甘婷婷. 基于叶绿素荧光的浮游植物生长状态监测技术[J]. 大气与环境光学学报, 2014, 9(3): 201. LIU Jing, LIU Wen-Qing, ZHAO Nan-Jing, ZHANG Yu-Jun, MA Ming-Jun, DAI Pang-Da, YIN Gao-Fang, DUAN Jing-Bo, YU Xiao-Ya, FANG Li, GAN Ting-Ting. Monitoring Technique of Phytoplankton Growth State Based on Chlorophyll Fluorescence[J]. Journal of Atmospheric and Environmental Optics, 2014, 9(3): 201.

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