中国激光, 2009, 36 (10): 2498, 网络出版: 2009-10-23   

光学相干层析术在人体皮肤成像方面的实验研究

Experiment Research on Optical Coherence Tomography of Human Skin
作者单位
南京理工大学 电光学院光学工程系,江苏 南京 210094
摘要
光学相干层析术(OCT)在人体皮肤病的临床诊断及科学研究方面有着广阔的应用前景。为获得活体状态下人体皮肤形态结构图像及其散射特性参数,研制了一套OCT系统(16 μm纵向分辨率,-90 dB的系统灵敏度),应用该系统对人体多个部位的皮肤进行了活体成像,基于扩展的Huygens-Fresnel原理推导了OCT外差信号的表达式,利用该表达式对不同区域的OCT纵向信号曲线段进行线性拟合,提取人体皮肤的散射系数。从获得的结构图像可以清晰地分辨出角质层(掌趾部位)、表皮层、真皮上层等皮肤结构,分析得到了角质层和表皮层的厚度信息以及散射系数。从而验证了OCT用于表层皮肤研究的可行性。
Abstract
Optical coherence tomography (OCT) is a promising new imaging method for clinical diagnosis and scientific research. In order to obtain the morphologic structure and the scattering coefficient of in vivo human skin,an OCT system with 16 μm axial resolution and -90 dB detection sensitivity is created. With this system in vivo OCT image of human skin in several locations is formed. And the heterodyne signal expression is deduced from the OCT model based on the extended Huygens-Fresnel principle. Through the fit of heterodyne signal expression to the various parts of the OCT axial signal curve,the scattering coefficients is acquired. Structures of the stratum corneum (plamoplantar),the epidermis,and the upper dermis can be identified from the obtained cross-sectional images. The thickness of the stratum corneum and the epidermis are measured,as well as the scattering coefficients. It is demonstrated that OCT is a feasible method for investigation of morphologic structure of superficial layers of human skin.
参考文献

[1] D. Huang,E. A. Swanson,C. P. Lin et al.. Optical coherence tomography[J]. Science,1991,254(5035):1178-1181

[2] 杨亚良,丁志华,孟 婕 等. 适合于内窥成像的共路型光学相干层析成像系统[J]. 光学学报,2008,28(5):955-959

    Yang Yaliang,Ding Zhihua,Meng Jie et al.. Common path optical coherence tomographic system suitable for endoscopic imaging[J]. Acta Optica Sinica,2008,28(5):955-959

[3] M. R. Hee,J. A. Izatt,E. A. Swanson et al.. Optical coherence tomography of the human retina[J]. Arch. Ophthalmol.,1995,113(3):325-332

[4] M. R. Hee,C. A. Puliafito,C. Wong et al.. Optical coherence tomography of macular holes[J]. Ophthalmology,1995,102(5):748-756

[5] 王玲,丁志华,史国华 等. 基于快速扫描延迟线相位调制的光纤型光学相干层析系统[J]. 中国激光,2008,35(3):472-476

    Wang Ling,Ding Zhihua,Shi Guohua et al.. Fiber-based optical coherence tomography imaging system with rapid scanning optical delay line as phase modulator[J]. Chinese J. Lasers,2008,35(3):472-476

[6] 史国华,丁志华,戴 云 等. 光纤型光学相干层析技术系统的眼科成像[J]. 中国激光,2008,35(9):1429-1431

    Shi Guohua,Ding Zhihua,Dai Yun et al.. Ophthalmic imaging by optical coherence tomography[J]. Chinese J. Lasers,2008,35(9):1429-1431

[7] 张雨东,戴 云,史国华 等. 一维小波变换在时域光学相干层析成像中的应用[J]. 中国激光,2008,35(7):1013-1016

    Zhang Yudong,Dai Yun,Shi Guohua et al.. Application of one-dimensional wavelet transformation in time domain optical coherence tomography imaging[J]. Chinese J. Lasers,2008,35(7):1013-1016

[8] J. M. Schmitt,M. J. Yadlowsky,R. F. Bonner. Subsurface imaging of living skin with optical coherence microscopy[J]. Dermatology,1995,191(2):93-98

[9] J. Welzel. Optical coherence tomography in dermatology:a review[J]. Skin Res. Technol.,2001,7(1):1-9

[10] T. Gambichler,G. Moussa,M. Sand et al.. Applications of optical coherence tomography in dermatology[J]. J. Dermatol. Sci.,2005,40(2):85-94

[11] T. Gambichler,A. Orlikov,R. Vasa et al.. In vivo optical coherence tomography of basal cell carcinoma[J]. J. Dermatol. Sci.,2007,45(3):167-173

[12] J. M. Olmedo,K. E. Warschaw,J. M. Schmitt et al.. Correlation of thickness of basal cell carcinoma by optical coherence tomography in vivo and routine histologic findings:a pilot study[J]. Dermatol. Surg.,2007,33(4):421-425;discussion 425-426

[13] T. Gambichler,B. Kunzlberger,V. Paech et al.. UVA1 and UVB irradiated skin investigated by optical coherence tomography in vivo:a preliminary study[J]. Clin. Exp. Dermatol.,2005,30(1):79-82

[14] T. Gambichler,R. Matip,G. Moussa et al.. In vivo data of epidermal thickness evaluated by optical coherence tomography:effects of age,gender,skin type,and anatomic site[J]. J. Dermatol. Sci.,2006,44(3):145-152

[15] T. Gambichler,S. Boms,M. Stucker et al.. Epidermal thickness assessed by optical coherence tomography and routine histology:preliminary results of method comparison[J]. J. Eur. Acad. Dermatol. Venereol.,2006,20(7):791-795

[16] J. M. Schmitt,A. Knüttel,R. F. Bonner. Measurement of optical properties of biological tissues by low-coherence reflectometry[J]. Appl. Opt.,1993,32(30):6032-6042

[17] J. M. Schmitt,A. Knuttel,M. Yadlowsky et al.. Optical-coherence tomography of a dense tissue:statistics of attenuation and backscattering[J]. Phys. Med. Biol.,1994,39(10):1705-1720

[18] A. Knüttel,M. Boehlau-Godau. Spatially confined and temporally resolved refractive index and scattering evaluation in human skin performed with optical coherence tomography[J]. J. Biomed. Opt.,2000,5(1):83-92

[19] D. Levitz,L. Thrane,M. Frosz et al.. Determination of optical scattering properties of highly-scattering media in optical coherence tomography images[J]. Opt. Express,2004,12(2):249-259

[20] 王凯,丁志华,王 玲. 基于光学相干层析术的组织光学性质测量[J]. 光子学报,2008,37(3):523-527

    Wang Kai,Ding Zhihua,Wang Ling. Measuring tissue optical properties by optical coherence tomography[J]. Acta Photonica Sinica,2008,37(3):523-527

[21] L. Thrane,H. T. Yura,P. E. Andersen. Analysis of optical coherence tomography systems based on the extended Huygens-Fresnel principle[J]. J. Opt. Soc. Am. A,2000,17(3):484-490

[22] 李鹏,高万荣. 光学相干层析系统的信噪比分析及优化[J]. 中国激光,2008,35(4):635-640

    Li Peng,Gao Wanrong. Signal-to-noise ratio analysis and optimization of optical coherence tomographic imaging system[J]. Chinese J. Lasers,2008,35(4):635-640

[23] G. Vargas,E. K. Chan,J. K. Barton et al.. Use of an agent to reduce scattering in skin[J]. Lasers Surg. Med.,1999,24(2):133-141

[24] A. G. Podoleanu. Unbalanced versus balanced operation in an optical coherence tomography system[J]. Appl. Opt.,2000,39(1):173-182

李鹏, 黄润, 高万荣. 光学相干层析术在人体皮肤成像方面的实验研究[J]. 中国激光, 2009, 36(10): 2498. Li Peng, Huang Run, Gao Wanrong. Experiment Research on Optical Coherence Tomography of Human Skin[J]. Chinese Journal of Lasers, 2009, 36(10): 2498.

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