激光与光电子学进展, 2019, 56 (15): 151701, 网络出版: 2019-08-05 复制并引用该论文  被引通知

图 & 表

图 1. 细胞培养天数与细胞存活率的趋势

Fig. 1. Trends in cell culture days and cell viability

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图 2. 光学显微镜观察A549和Met-5A两种细胞随培养时间的延长生长形态学特征。(a) A549;(b) Met-5A

Fig. 2. Morphological characteristics of A549 and Met-5A cells in optical microscopy with extension of culture time. (a) A549; (b) Met-5A

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图 3. 拉曼仪器下的A549和Met-5A细胞光学图像。(a) A549;(b) Met-5A

Fig. 3. Optical images of A549 and Met-5A cells under Raman instruments. (a) A549; (b) Met-5A

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图 4. A549和Met-5A细胞的拉曼位移特征比较。实箭头指示1082 cm-1拉曼峰,虚箭头指示1155 cm-1拉曼峰

Fig. 4. Comparison of Raman shift characteristics between A549 and Met-5A cells. Solid arrow indicates Raman peak at 1082 cm-1, and dotted arrow indicates Raman peak at 1155 cm-1

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图 5. 用PAP和H&E分别染色A549和Met-5A的结果。(a)(b)(e)(f) A549;(c)(d)(g)(h) Met-5A

Fig. 5. Staining results of A549 and Met-5A cells with PAP and H&E, respectively. (a)(b)(e)(f) A549; (c)(d)(g)(h) Met-5A

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表 1拉曼光谱的特征峰值分配

Table1. Characteristic peak assignments for Raman spectra

Peak position /cm-1 Majorassignments PAPstain affinity H&E stainaffinity 786 DNA:O-P-O + + 1082 O-P-O- stretch + + 1155 C-N stretching (protein) ++ + 1258 Amide III - - 1304 CH2 deformation (lipid) - - 1450 CH2CH3 twistingmode(lipid) - - 1658 Amide I (C=O stretchingmode of proteins,a-helix conformation) - - Note: + and++ indicate affinity degree of H&E and PAP dyes with corresponding chemical groups; - indicates that H&E and PAP dyes are negatively colored with corresponding chemical groups.

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表 2两种染料分别染色A549和Met-5A两种类型细胞的图像特征参数

Table2. Image characteristic parameters of A549 and Met-5A cells stained by two dyes

Characteristic parameter Cell PAP (n=100) H&E (n=100) Energy variance A549 7.91419±1.66525 7.98664±1.68421 Met-5A 4.62469±1.37511 8.16944±1.83352 t 10.771 -0.519 p <0.001 0.605 Color mean A549 7.06792±0.04892 7.11570±0.04107 Met-5A 7.10321±0.03602 7.05825±0.02611 t -4.109 8.347 p <0.001 <0.001

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杨玉, 陶正荣, 闫静, 吕殊星, 王然, 刘晓丹, 孙续国. 肺癌细胞物质表达及堆积结构的拉曼位移特征[J]. 激光与光电子学进展, 2019, 56(15): 151701. Yu Yang, Zhengrong Tao, Jing Yan, Shuxing Lü, Ran Wang, Xiaodan Liu, Xuguo Sun. Raman Shift Characteristics of Substance Expression and Accumulation Structure in Lung Cancer Cells[J]. Laser & Optoelectronics Progress, 2019, 56(15): 151701.