靶向量子点的合成及其在活体成像研究中的应用

翟鹏; 许改霞; 朱小妹; 王晓梅; 牛憨笨

doi:doi:10.3788/cjl201340.0104003

中国激光, 2013, 40 (1): 0104003, 网络出版: 2012-12-13

靶向量子点的合成及其在活体成像研究中的应用

Synthesis of Targeting Quantum Dot and Its Applications in In Vivo Imaging Research

论文大纲

翟鹏 ^1,2,*许改霞 ^1,2朱小妹 ¹王晓梅 ²牛憨笨 ¹

作者单位

¹ 深圳大学光电工程学院，教育部/广东省光电子器件与系统重点实验室，广东深圳 518060

² 深圳大学医学院，深圳市生物医学工程重点实验室，广东深圳 518060

摘要

利用水相法制备CdTe量子点，在不同细胞培养基及不同pH值环境中对其进行了稳定性表征，研究了量子点对HeLa细胞增殖抑制率的影响，而后利用耦联转铁蛋白的量子点对HeLa细胞进行了靶向性标记，最后将量子点与葡聚糖耦联，通过透明背脊皮翼视窗观察其在血管内的动态过程。结果表明：合成的量子点发射谱峰值为660 nm，在DMEM和M1640细胞培养基中具有良好稳定性，当缓冲液pH值由5升高到13时，量子点荧光强度先升高后下降；量子点浓度为13 μg/mL时，HeLa细胞存活率高于80%；耦联转铁蛋白的量子点对HeLa细胞靶向作用明显；可观察到耦联葡聚糖的量子点在小鼠血管中的动态运动。该研究表明合成的量子点可成功用于活体成像。

Abstract

We synthesize CdTe quantum dots in aqueous solution and characterize the stability of quantum dots in different culture media and different pH environments. The impact of quantum dots on HeLa cellular proliferation inhibition rate is studied. The quantum dots conjugated with transferrin are used for HeLa cell labelling. Finally, the quantum dots are conjugated with dextran and applied in the transparent dorsal skin fold window chamber to observe the dynamics of the bioconjugations in blood vessels. The experimental results show that the quantum dots peaking at 660 nm are stable in cell culture media of DMEM and M1640. When the pH of the buffer solutions increases from 5 to 13, the fluorescence intensity of CdTe increases firstly and then reduces. The cell viability is higher than 80% even when the concentration of quantum dots is 13 μg/mL. The quantum dots conjugated with transferrin obviously target to the HeLa cells. What′s more, the clear dynamic flourescence images of quantum dots conjugated with dextran are observed under microscope. The study shows that such quantum dots can be used for living imaging research successfully.

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翟鹏, 许改霞, 朱小妹, 王晓梅, 牛憨笨. 靶向量子点的合成及其在活体成像研究中的应用[J]. 中国激光, 2013, 40(1): 0104003. Zhai Peng, Xu Gaixia, Zhu Xiaomei, Wang Xiaomei, Niu Hanben. Synthesis of Targeting Quantum Dot and Its Applications in In Vivo Imaging Research[J]. Chinese Journal of Lasers, 2013, 40(1): 0104003.

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