激光生物学报, 2011, 20 (2): 269, 网络出版: 2015-10-08  

贴壁培养细胞台盼蓝拒染试验的方法学探讨

A Methodological Study of Trypan Blue Exclusion Test for Cultured Adherent Cells
作者单位
湖南师范大学生命科学学院, 湖南 长沙 410081
引用该论文

彭彬, 吴晶晶, 李烨, 徐倩, 唐琳, 汪保和. 贴壁培养细胞台盼蓝拒染试验的方法学探讨[J]. 激光生物学报, 2011, 20(2): 269.

PENG Bin, WU Jing-jing, LI Ye, XU Qian, TANG Lin, WANG Bao-he. A Methodological Study of Trypan Blue Exclusion Test for Cultured Adherent Cells[J]. Acta Laser Biology Sinica, 2011, 20(2): 269.

参考文献

[1] MA Y, YUN S R, NAM S Y, et al. Protective Effects of Sanjoinine Against N-methyl-D-aspartate-induced Seizure[J]. Biol Pharm Bull, 2008, 31(9): 1749-1754.

[2] TAO R, ZHANG J, VESSEY D A, et al. Deletion of the Sphingosine Kinase-1 Gene Influences Cell Fate during Hypoxia and Glucose Deprivation in Adult Mouse Cardiomyocytes[J]. Cardiovasc Res, 2007, 74(1): 56-63.

[3] LAISHES B A, WILLIAMS G M.Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. II. Dexamethasone Enhanced Longevity and Maintenance of Morphology[J]. In Vitro, 1976, 12(12): 821-832.

[4] MISHRA V, SAXENA D K, DAS M. Effect of Argemone Oil and Argemone Alkaloid, Sanguinarine on Sertoli-germ Cell Coculture[J]. Toxicol Lett, 2009, 186(2) : 104-110.

[5] 朱海林, 彭彬, 吴晶晶, 等.培养板单层贴壁细胞的原位计数方法[J].细胞生物学杂志, 2009, 31(5): 719-725.

    ZHU Hai-lin, PENG bin, WU Jing-jing, et al. A Counting Method for Monolayer Cells Attached to Culture Plate in Situ[J]. Cytobiology, 2009, 31(5): 719-725.

[6] STROBER W. Trypan Blue Exclusion Test of Cell Viability[J]. Curr Protoc Immunol, 2001, Appendix 3: Appendix 3B.

[7] WU H, YANG J M, JIN S, et al. Elongation Factor-2 kinase Regulates Autophagy in Human Glioblastoma Cells[J]. Cancer Res, 2006, 66(6): 3015-3023.

[8] BUEHRING G C, JENSEN H M. Lack of Toxicity of Methylene Blue Chloride to Supravitally Stained Human Mammary Tissues[J]. Cancer Res, 1983, 43(12 Pt 1): 6039-6044.

[9] FERNYHOUGH M E, HELTERLINE D L, VIERCK J L, et al. Coulter Counter Use in the Enumeration of Muscle and Fat Stem Cells[J]. Methods Cell Sci, 2003, 25(3-4): 221-225.

[10] PERRY S W, EPSTEIN LG, GELLBARD H A. In Situ Trypan Blue Staining of Monolayer Cell Cultures for Permanent Fixation and Mounting[J]. Biotechniques, 1997, 22(6): 1020-1021.

[11] MACHIDA-SANO I, MATSUDA Y, NAMIKI H. A Novel Harvesting Method for Cultured Cells using Iron-cross-linked Alginate Films as Culture Substrates[J]. Biotechnol Appl Biochem, 2009, 55(1): 1-8.

彭彬, 吴晶晶, 李烨, 徐倩, 唐琳, 汪保和. 贴壁培养细胞台盼蓝拒染试验的方法学探讨[J]. 激光生物学报, 2011, 20(2): 269. PENG Bin, WU Jing-jing, LI Ye, XU Qian, TANG Lin, WANG Bao-he. A Methodological Study of Trypan Blue Exclusion Test for Cultured Adherent Cells[J]. Acta Laser Biology Sinica, 2011, 20(2): 269.

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