基于图像拼接的高通量数字PCR荧光基因芯片读取系统的设计

针对高通量数字聚合酶链式反应荧光基因芯片检测的需求，提出了一种基于荧光显微光学技术的基因芯片检测系统。系统以无限远荧光显微系统为框架，通过制冷CCD一次完成较大视场的成像，顺序移动基因芯片得到全部图像，通过图像拼接完成检测，切换二向色镜组实现检测不同荧光通道的目的。光学系统分辨率可达16.3μm、曝光时间500ms，目前只需要拼接35次，即可在1min内完成对28mm×16mm的基因芯片内两万多荧光通道的检测，极大的提高了检测效率。

Abstract

In face of the requirement of the detection of high throughput digital polymerase chain reaction (dPCR) fluorescence gene chip, a new optical detection system based on fluorescence microscopy is proposed. The design of this system is depending on an afocal fluorescence microscopy system. It can complete the imaging of the larger field of view through refrigerating CCD. All the images can be got by moving the gene chip in sequence, and the complete image can be obtained through image splicing. By changing the dichroic mirror and filter, the detection system can detect different kinds of fluorescence gene chip. The optical system has a resolution of 20mm, an exposure time of 500ms and 35 times of splicing. It can complete the detection of the gene chip with more than 20000 channel within 1 minutes, and the area of the gene chip is 28mm×18mm. This system greatly reduces the detection time.

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朱天赟, 郑继红, 孙刘杰, 万新军, 黄新荣. 基于图像拼接的高通量数字PCR荧光基因芯片读取系统的设计[J]. 光学技术, 2019, 45(1): 107. ZHU Tianyun, ZHENG Jihong, SUN Liujie, WAN Xinjun, HUANG Xinrong. Design of high throughput digital PCR fluorescence gene chip detection system based on image mosaic[J]. Optical Technique, 2019, 45(1): 107.

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