中国激光, 2018, 45 (2): 0207014, 网络出版: 2018-02-28
多光子显微技术在医学诊断中的应用 
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Application of Multiphoton Microscopy in Disease Diagnosis
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图 2. 人体食管基质的(a) SHG图像,(b) TPEF图像,(c) SHG(灰色)/TPEF(绿色)复合图像[25](激发波长为850 nm,标尺为20 μm)
Fig. 2. (a) SHG, (b) TPEF, (c) combined SHG (grey) and TPEF (green) images of the human esophageal stroma[25] (excitation wavelength is 850 nm, scale bar is 20 μm)
图 3. 癌变的胃黏膜和黏膜下层的典型TPEF/SHG复合图像[26](标尺为50 μm)
Fig. 3. Representative TPEF/SHG images of cancerous gastric mucosa and submucosa[26](scale bar is 50 μm)
图 4. (a)正常,(b)非侵入瘤变,(c)侵入瘤变的结肠柱状上皮的典型TPEF图像[19](标尺为50 μm)
Fig. 4. (a) Representative multiphoton images of normal, (b) precancerous, (c) cancerous colonic tissues at depth of 0 μm[19](scale bar is 50 μm)
图 5. (a)正常,(b)非侵入瘤变,(c)侵入瘤变结肠柱状上皮的典型 SHG图像(激发波长为800 nm[22],图像尺寸为415 μm×490 μm,标尺为50 μm)
Fig. 5. (a) Representative SHG images of normal, (b) precancerous, (c) cancerous colonic tissues[22] (excitation wavelength is 800 nm, image size is 415 μm×490 μm, scale bar is 50 μm)
图 6. (a)不同年龄(20、40、70岁)病人离体真皮的MPM图像(蓝色代表SHG信号,绿色代表TPEF信号);(b)皮肤老化程度的SAAID指数图[42]
Fig. 6. (a) SHG and multiphoton imaging of ex-vivo human dermis in 20, 40, and 70 years-old patients (blue is SHG and green is TPEF); (b) skin aging quantification of these tissues using SAAID metric[42]
图 7. (a)基于MPM的离体人体基底细胞癌光诊断(蓝色代表SHG信号,绿色代表TPEF信号);(b)相邻切片的H&E染色图[44]
Fig. 7. (a) Optical diagnosis of ex-vivo human basal cell carcinoma using MPM imaging (blue is SHG, green is TPEF); (b) adjacent H & E histological section[44]
图 8. 整个GFP小鼠角膜的MPM图像(绿色代表TPEF信号,蓝色代表SHG信号[53])
Fig. 8. Multiphoton image across entire cornea of GFP mouse (green is TPEF, blue is SHG [53])
林宏心, 左宁, 卓双木, 陈建新. 多光子显微技术在医学诊断中的应用[J]. 中国激光, 2018, 45(2): 0207014. Lin Hongxin, Zuo Ning, Zhuo Shuangmu, Chen Jianxin. Application of Multiphoton Microscopy in Disease Diagnosis[J]. Chinese Journal of Lasers, 2018, 45(2): 0207014.
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