光纤式微液滴光学检测与计数单元仿真分析

为实现微流控芯片上微液滴的检测与计数, 设计了光纤式检测与计数单元, 使用TracePro软件进行建模仿真以便为检测信号处理提供依据。根据液滴通过检测区域时引起的光强变化来实现计数, 分析了照明光束准直性、液滴尺寸、液滴相对溶液折射率以及接收光纤相距芯片距离对光强变化的影响。仿真实验结果表明, 照明光束准直性越差, 液滴半径越大, 相对溶液折射率越低, 接收光纤距离越近, 相对光强变化越明显, 并且液滴大小决定着光强变化曲线中是否会出现双波谷现象, 液滴半径小于13 μm时, 液滴检测信号为明显的单波谷, 半径大于17 μm时, 液滴信号为明显双波谷。根据仿真结果提出了检测信号的处理方法, 表明该单元可以实现微流控芯片上微液滴的检测与计数功能。

Abstract

A detection and counting unit with optical fiber was designed for realizing the detection and counting of micro-droplets on the microfluidic chip. TracePro was used to simulate and provide the basis for detection signal processing. The system can realize the counting function according to the change of the optical intensity, which is caused by droplet passing. Simulation were carried out to analyze the influence of beam collimation, droplet size, droplet refractive index and distance from receiving fiber to chip on optical intensity. The results obtained from the simulation show that the poorer the collimation beam, the larger the droplet radius, the smaller the refractive index, and the closer the distance between receiving fiber and chips, which can cause more apparent variation in optical intensity. What’s more, the droplet size determines whether there are double valleys in the optical intensity curve. When the radius of droplet is less than 13 μm, there is one valley in the optical intensity curve. And when the radius is larger than 17 μm, there are two valleys.In addition, processing method of detecting signal was proposed based on the simulation results, showing that this optical detection unit can realize the detection and counting function of the micro-droplets on the microfluidic chip.

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郎明远, 张林, 曹振忠, 黄战华. 光纤式微液滴光学检测与计数单元仿真分析[J]. 应用光学, 2019, 40(1): 172. LANG Mingyuan, ZHANG Lin, CAO Zhenzhong, HUANG Zhanhua. Simulation and analysis of micro-droplet optical detection and counting unit with optical fiber[J]. Journal of Applied Optics, 2019, 40(1): 172.

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