激光生物学报, 2017, 26 (1): 91, 网络出版: 2017-04-10  

一种体外快速分离脂肪来源干细胞的方法

One Easy Method to Isolate Adipose-Derived Stem Cells in vitro
郭淑军 1,2,3,4,5,*刘伟 1,2,3,4,5金媛 1,2,3,4,5王强 1,2,3,4,5李雅丹 1,2,3,4,5陈小佳 1,2,3,4,5
作者单位
1 暨南大学生物医药研究院, 广东 广州 510632
2 暨南大学细胞生物学系, 广东 广州 510632
3 基因工程药物国家工程研究中心, 广东 广州 510632
4 广东省生物工程药物重点实验室, 广东 广州 510632
5 广东省生物技术药物工程技术研究中心, 广东 广州 510632
引用该论文

郭淑军, 刘伟, 金媛, 王强, 李雅丹, 陈小佳. 一种体外快速分离脂肪来源干细胞的方法[J]. 激光生物学报, 2017, 26(1): 91.

GUO Shujun, LIU Wei, JIN Yuan, WANG Qiang, LI Yadan, CHEN Xiaojia. One Easy Method to Isolate Adipose-Derived Stem Cells in vitro[J]. Acta Laser Biology Sinica, 2017, 26(1): 91.

参考文献

[1] AUGELLO A, KURTH T B, DE BARI C. Mesenchymal stem cells:a perspective from in vitrocultures to in vivo migration and niches[J].Eur Cell Mater, 2010, 20:121-133.

[2] MIN GUAN, WEI YAO, RUIWU LIU, et al. Directing mesenchymal stem cells to bone to augment bone formation and increase bone mass[J].NAT MED, 2012, 18(3):456-462

[3] YAMAMOTO N, AKAMATSU H, HASEGAWA S, et al. Isolation of multipotent stem cells from mouse adiposetissue [J]. J Dermatol Sci, 2007, 48(1): 43-52.

[4] TROPEL P, NOL D, PLATET N, et al.Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow[J].Exp Cell Res, 2004, 295(2):395-406.

[5] ZHU Y, LIU T, SONG K, et al. Adipose-derived stem cell:a better stem cell than BMSC[J]. Cell Biochem Funct, 2008, 26(6):664-675.

[6] BENSIDHOUM M, CHAPEL A, FRANCOIS S, et al. Homing of in vitro expanded Stro-1-or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment [J]. Blood, 2004, 103(9): 3313-3319.

[7] ZUK P A, ZHU M, MIZUNO H, et al. Multilineage cells from human adipose tissue:implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2):211-228.

[8] 陈犹白, 陈聪慧, ZHANG Qixu, 等.脂肪干细胞分离、纯化和保存:研究进展与未来方向[J].中国组织工程研究, 2016, 20(10):1508-1520.

    CHEN Youbai, CHEN Conghui, ZHANG Qixu, et al. Isolation, purification and preservation of adipose-derived stem cells: research progress and future development[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1508-1520.

[9] 吴尉, 梁芳, 宋小琴, 等.人脂肪干细胞的提取和鉴定[J].中国组织工程研究, 2015, 19(28):4498-4502.

    WU Wei, LIANG Fang, SONG Xiaoqin, et al. Extraction and identification of human adipose-derived stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28):4498-4502.

[10] DE UGARTE D A, MORIZONO K, ELBARBARY A, et al.Comparison of multi-lineage cells from human adipose tissue and bone marrow[J].Cells Tissues Organs, 2003, 174(3):101-109.

[11] WICKHAM M Q, ERICKSON G R, GIMBLE J M, et al.Multipotent stromal cells derived from the infrapatellar fat pad of the knee[J].Clin Orthop Relat Res, 2003, (412):196-212.

[12] GAUSTAD K G, BOQUEST A C, ANDERSON B E, et al.Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes[J].Biochem Biophys Res Commun, 2004, 314(2)420-427.

[13] 刘晓潭, 徐海斌, 路坦.小鼠脂肪来源干细胞向成骨及软骨细胞的诱导分化[J].中国组织工程研究, 2013, 17(14):2525-2531.

    LIU Xiaotan, XU haibin, LU Tan. Adipose-derived stem cells differentiate into osteoblasts and chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14):2525-2531.

[14] RODRIGUEZ A M, ELABD C, AMRI E Z, et al.The human adipose tissue is a source of multipotent stem cells[J].Biochimie, 2005, 87(1):125-128.

[15] BOQUEST A C, SHAHDADFAR A, FRNSDAL K, et al. Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture[J]. Mol Biol Cell, 2005, 16(3):1131-1141.

[16] ZUK P A, ZHU M, ASHJIAN P, et al. Human adipose tissue is a source of multipotent stem cells[J]. Mol Biol Cell, 2002, 13(12):4279-4295.

[17] LEI L, LIAO W M, SHENG P Y, et al. Biological character of human adipose-derived adult stemcells and influence of donor age on cell replication in culture [J]. Sci China C Life Sci, 2007, 50(3):320-328.

[18] VIEIRA M, BRANDALISE V, ZUCCONI E, et al. Isolation, characterization and differentiation potential of canine adipose-derived stem cells [J]. Cell Transplant, 2010, 19(3):279-289.

[19] HORN P, BORK S, WAGNER W. Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis[J]. Cyotherapy, 2009, 10(7): 676-685.

[20] YOSHIMURA K, SHIGEURA T, MATSUMOTO D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates [J]. J CELL PHYSIOL, 2006, 208(1): 64-76.

[21] KO M S, JUNG J Y, SHIN S I, et al. Effects of expanded human adipose tissue-derived mesenchymal stem cells on the viability of cryopreserved fat grafts in the nude mouse [J]. Int J Med Sci, 2011, 8(3): 231-238.

[22] LIN G, GARCIA M, NING H, et al.Defining stem and progenitor cells within adipose tissue[J].Stem Cells Dev, 2008, 17(6)1053-1063.

[23] LIN K, MATSUBARA Y, MASUDA Y, et al.Characterization of adipose tissue-derived cells isolated with the celution system[J].Cytotherapy, 2008, 10(4):417-426.

[24] VARMA M J, BREULS R G, SCHOUTEN T E, et al.Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells[J].Stem Cells Dev, 2007, 16(1):91-104.

郭淑军, 刘伟, 金媛, 王强, 李雅丹, 陈小佳. 一种体外快速分离脂肪来源干细胞的方法[J]. 激光生物学报, 2017, 26(1): 91. GUO Shujun, LIU Wei, JIN Yuan, WANG Qiang, LI Yadan, CHEN Xiaojia. One Easy Method to Isolate Adipose-Derived Stem Cells in vitro[J]. Acta Laser Biology Sinica, 2017, 26(1): 91.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!