中国激光, 2020, 47 (11): 1107001, 网络出版: 2020-11-02
基于显微镜数字图像的荧光免疫层析定量检测方法 下载: 863次
Quantitative Detection Method of Fluorescence Immunochromatography Based on Microscopic Digital Image
图 & 表
图 4. 面阵CCD成像原理示意图。(a) CCD成像原理简图;(b) RGB亮度阶梯
Fig. 4. Schematics of area array CCD imaging. (a) Schematic of CCD imaging principle; (b) RGB brightness ladder
图 5. RGB像元亮度直方图。(a) R分量像元亮度直方图;(b) G分量像元亮度直方图;(c) B分量像元亮度直方图
Fig. 5. RGB component pixels brightness histogram. (a) R component pixel brightness histogram; (b) G component pixel brightness histogram; (c) B component pixel brightness histogram
图 7. 荧光免疫反应原理。(a)待测溶液的制备;(b)试纸结构
Fig. 7. Principle of fluorescence immunity reaction. (a) Preparation of test solution; (b) structure of test paper
图 8. 不同浓度尿孕酮(P)分子的荧光信号强度曲线
Fig. 8. Fluorescence signal intensity curves of urine progesterone (P) molecule with different mass concentrations
表 1CCD相关参数
Table1. CCD related parameters
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表 2拟合结果
Table2. Fitting result
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李婷婷, 冯大伟, 李琦, 向阳, 贾强, 路雨桐, 赵阳. 基于显微镜数字图像的荧光免疫层析定量检测方法[J]. 中国激光, 2020, 47(11): 1107001. Li Tingting, Feng Dawei, Li Qi, Xiang Yang, Jia Qiang, Lu Yutong, Zhao Yang. Quantitative Detection Method of Fluorescence Immunochromatography Based on Microscopic Digital Image[J]. Chinese Journal of Lasers, 2020, 47(11): 1107001.