温度对奶粉荧光光谱的影响

吴彬青; 叶满萍; 史亚荣; 郑枭; 赵春柳; 陈华才

doi:doi:10.3788/gzxb20164507.0730004

光子学报, 2016, 45 (7): 070730004, 网络出版: 2016-08-18

温度对奶粉荧光光谱的影响

Effect of Temperature on Fluorescence Spectra of Milk Powder

论文大纲

吴彬青 ^*叶满萍史亚荣郑枭赵春柳陈华才

作者单位

中国计量学院光学与电子科技学院, 杭州 310018

荧光光谱荧光寿命奶粉酪蛋白温度氨基酸 Fluorescence spectroscopy Fluorescence lifetime Milk Casein Temperature Amino acids

摘要

采用荧光光谱和荧光寿命技术研究了温度对奶粉荧光特征的影响.通过比较奶粉与酪蛋白、乳糖及维生素的荧光光谱, 确定了奶粉的荧光主要来自酪蛋白的贡献.随着温度的升高, 奶粉和酪蛋白的荧光强度减小, 其中幼儿配方奶粉和成人普通奶粉的荧光强度随温度升高减小的趋势基本相同, 而婴儿配方奶粉的荧光强度随温度的升高出现了一个相对变化缓慢的平台区, 酪蛋白的荧光强度随温度升高呈缓慢下降的趋势, 下降的速度比奶粉的要缓.酪蛋白加热到90℃然后冷却至25℃, 荧光强度部分恢复, 说明一部分蛋白质的结构变化或者荧光基团的活性变化是可逆的.测量了不同温度下奶粉和酪蛋白的荧光寿命.奶粉和酪蛋白的荧光寿命曲线在双指数拟合之后, 都有两个寿命, 这两个寿命可能来自于色氨酸和苯丙氨酸.随着温度的升高, 奶粉和酪蛋白的两个荧光寿命都减小.

Abstract

The effect of temperature on the fluorescence of milk powder was studied by fluorescence spectra and fluorescence lifetime technology. Firstly, the fluorescence of milk powder is mainly from casein determined by comparing the fluorescence spectra of milk powder, casein, lactose and vitamins. Secondly, as the temperature increases, the fluorescence intensity of infant formula powder, toddler formula powder, adult ordinary milk powder and casein are generally reduced. The trend of fluorescence intensity with temperature of toddler formula powder and adult ordinary milk is almost same. The change of the fluorescence intensity of infant formula powder with temperature is different, which shows a relatively slow-changing platform area. Casein′s fluorescence strength is a slow downward with increasing temperature, falling slower than milk. When heated to 90 degrees and then cooled to 25 degrees, casein was partially recovered, indicating that changes in the activity of the protein structural or fluorophore are partially reversible. Finally, the fluorescence lifetime of milk powder and casein at different temperatures were measured. Milk powder and casein have two lifetimes after double exponential fit, which can be derived from tryptophan and phenylalanine. As the temperature increases, fluorescence lifetimes of milk powder and casein are decreasing.

参考文献

[1] 顾春峰, 兰秀风, 于银山, 等.牛奶水溶液荧光光谱影响因素的研究［J］. 激光与光电子学进展 2012,49(03):130-135.

GU Chun-feng,LAN Xiu-feng,YU Yin-shan,et al. Research on influencing factors of milk aqueous solution fluorescence spectrum［J］. Laser and Optoelectronics Progress,2012 ,49(03) : 130-2012.

[2] 国晓炜, 李丽华, 张金生, 等. FT-IR法测定温度对牛奶脂肪酸结构的影响［J］. 食品研究与开发, 2012,33(05):178-180.

GUO Xiao-wei,LI Li-hua,ZHANG Jing-sheng,et al.Effect of temperature on structure of fatty acids in milk by FT-IR［J］.Food Research and Development,2012,33(05):178-180.

[3] 谷玲.基于荧光光谱技术对牛奶的成分分析［D］. 无锡:江南大学,2011.

GU Ling. Based on the fluorescence spectroscopy of composition analysis of milk［D］.Wuxi: Jiangnan University,2011.

[4] 周睿.温度对奶粉类食品红外光谱的影响研究［D］. 杭州:中国计量学院,2014.

ZHOU Rui.Temperature effect on FT-MIR spectra of milk powder products［D］.Hangzhou:China Jiliang University,2014.

[5] 丁志群, 王金霞, 赵红霞, 等.基于三维荧光光谱技术的食用油快速分析研究［J］.光子学报,2015,44(3):125-129.

DING Zhi-qun,WANG Jin-xia,ZHAO Hong-xia,et al.Rapid analysing edible oil using three oimensional fluorescence spectrascopy［J］. Acta Photonice Sinica, 2015, 44(3):125-129.

[6] 兰秀风, 顾春峰, 于银山, 等.牛奶水溶液的偏振三维荧光光谱研究［J］. 光谱学与光谱分析, 2012,32(12):3281-3284.

LAN Xiu-feng,GU Chun-feng,YU Yin-shan,et al.Research on polarized three-dimensional fluorescence spectra of milk solution［J］. Spectroscopy and Spectral Analysis, 2012, 32(12): 3281-3284.

[7] 刘保生, 张丽惠, 李志云, 等.荧光光谱法研究拜复乐与小牛胸腺DNA的作用机理［J］.发光学报,2014,35(3):372-376.

LIU Bao-sheng, ZHANG Li-hui, LI Zhi-yun, et al. Studies on the interaction mechanism between moxifloxacin and ctDNA by fluorescence spectroscopy［J］.Chinese Journal of Luminescence, 2014,35(3):372-376.

[8] 王守业, 徐小龙, 刘清亮, 等.荧光光谱在蛋白质分子构象研究中的应用［J］. 化学进展, 2001,13(04):257-260.

WANG Shou-ye,XU Xiao-long,LIU Qing-liang,et al.The application of fluorescence spectroscopy in the study on protein conformation ［J］.Progress in Chemistry, 2001,13(4) : 257-260.

[9] 陆从文, 兰秀凤, 张林, 等.依巴斯汀与牛血清蛋白相互作用的荧光光谱研究［J］.光子学报,2015,44(10):1030004.

LU Cong-wen,LAN Xiu-feng,ZHANG Lin,et al.Interaction between ebastine and bovine serum albumin by fluorescence spectroscopy［J］. Acta Photonice Sinica, 2015, 44(10):1030004.

[10] 顾春峰, 兰秀风, 于银山, 等.牛奶水溶液的荧光光谱研究［J］. 光子学报, 2012,41(01):107-111.

GU Chun-feng,LAN Xiu-feng,YU Yinshan,et al.Fluorescence spectrum of milk solution［J］.Acta Photonice Sinica, 2012 ,41(01): 107-111.

[11] 常敏.牛奶质量光学检测的作用机理及影响因素研究［D］. 天津:天津大学,2007.

CHANG Min. Study on mechanism and influence factors of optical measurement of milk quality［D］.Tianjin:Tianjin University,2007.

[12] 李慧.某市市售婴儿配方奶粉中蛋白质和微量元素的监测及评价［J］. 现代预防医学, 2004,31(04):572-574.

LI Hui.The status of protein and trace elements in infant for mula from shenzhen markets［J］. Modern Preventive Medicine, 2004 ,31(4) : 572-574.

[13] 侯冬岩, 回瑞华, 李铁纯, 等.奶粉中蛋白质含量的光谱分析［J］.鞍山师范学院学报, 2004,6(04):37-39.

HOU Dong-yan, HUI Rui-hua, LI Tie-chun, et al.Determination of p rotein in milk powders［J］.Journal of Anshan Normal University, 2004 ,6(04): 37-39.

[14] 贾晓旭.婴幼儿奶粉关键营养素分析方法研究［D］. 苏州:苏州大学,2014.

JIA Xu-peng. Quality analysis study of key nutrients in infant milk powder［D］.Suzhou:Suzhou University,2014.

[15] 李东旭, 许潇, 李娜, 等.时间分辨荧光技术与荧光寿命测量［J］. 大学化学, 2008,23(04):1-11.

LI Dong-xu,XU Xiao,LI Na,et al.Time-resolved fluorescence technology and fluorescence lifetime measurement［J］.University Chemistry, 2008,23(04) : 1-11.

[16] 林燕金,黄衍堂,吴锦树,等.稀土荧光特性与温度关系［J］.光子学报,2016,45(01):0116002.

LIN Yan-jin,HUANG Yan-tang,WU Jin-shu,et al.Relationship between rare earth fluorescence characteristic and temperature［J］. Acta Photonice Sinica, 2016, 45(01):0116002.

[17] 郑枭, 叶满萍, 李裔.Pd(Ⅱ)与牛血清白蛋白相互作用的荧光光谱研究［J］. 中国计量学院学报,2015,26(01):60-64.

ZHEN Xiao,YE Man-ping,LI Yi.Fluorescence spectra of Pd(Ⅱ)-BSA(bovine serum album) solvent systems［J］. Journal of China Jiliang University, 2015, 26(01) : 60-64.

吴彬青, 叶满萍, 史亚荣, 郑枭, 赵春柳, 陈华才. 温度对奶粉荧光光谱的影响[J]. 光子学报, 2016, 45(7): 070730004. WU Bin-qing, YE Man-ping, SHI Ya-rong, ZHEN Xiao, ZHAO Chun-liu, CHEN Hua-cai. Effect of Temperature on Fluorescence Spectra of Milk Powder[J]. ACTA PHOTONICA SINICA, 2016, 45(7): 070730004.

温度对奶粉荧光光谱的影响

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