光学仪器, 2018, 40 (1): 13, 网络出版: 2018-04-09  

牛血清蛋白-Au配合物及其复合物制备及表征

Preparation and characterization of bovine serum albumin(BSA)-Au
王圣琼 1,2,3,*孙畅 4陶春先 1,2,3韩朝霞 1,2,3洪瑞金 1,2,3林辉 1,2,3张大伟 1,2,3
作者单位
1 上海理工大学 光电信息与计算机工程学院, 上海 200093
2 上海理工大学 教育部光学仪器与系统工程研究中心, 上海 200093
3 上海理工大学 上海市现代光学系统重点实验室, 上海 200093
4 复旦大学 高分子科学系, 上海 200433
摘要
以牛血清蛋白(bovine serum albumin,BSA)、氯金酸(HAuCl4)、NaOH等为原料,采用一锅合成法制备了牛血清蛋白-Au配合物(BSA-Au),观察到了样品的宽带红光荧光发射。通过库仑力作用将BSA-Au与表面带有正电荷的金纳米颗粒进行复合,对纳米复合物的吸收光谱及荧光光谱特性进行了研究。实验结果表明,在本实验条件下,金纳米颗粒虽然增强了复合物样品在可见光区域的光吸收,但对BSA-Au红光发光有猝灭作用。complexes and the Au nanoparticle@BSA-Au nanocomposites
Abstract
Bovine serum albumin(BSA)-Au complexes were synthesized by the one-pot method with BSA,HAuCl4 and NaOH as the starting materials.Red photoluminescence was observed from the sample.Au nanoparticle@BSA-Au composites were fabricated via the Coulomb interaction between the negatively charged BSA-Au and the positively charged Au nanoparticles.Optical absorption and photoluminesence properties of the samples were investigated.The experimental results show that optical absorption in the visible region was enhanced for the Au nanoparticle@BSA-Au composites,due to the localized surface plasmon resonance.However,the luminescence of BSA-Au was quenched.Further work on improving the structure of the Au nanoparticle@BSA-Au composites needs to be conducted.
参考文献

[1] CHEN L F,ZHANG Y Y,JIANG H,et al.Cytidine mediated AuAg nanoclusters as bright fluorescent probe for tumor imaging in vivo[J].Chinese Journal of Chemistry,2016,34(6):589-593.

[2] LIU Y L,AI K L,CHENG X L,et al.Gold-nanocluster-based fluorescent sensors for highly sensitive and selective detection of cyanide in water[J].Advanced Functional Materials,2010,20(6):951-956.

[3] LI L L,LIU H Y,SHEN Y Y,et al.Electrogenerated chemiluminescence of Au nanoclusters for the detection of dopamine[J].Analytical Chemistry,2011,83(3):661-665.

[4] ROYON A,BOURHIS K,BELLEC M,et al.Silver clusters embedded in glass as a perennial high capacity optical recording medium[J].Advanced Materials,2010,22(46):5282-5286.

[5] DE CREMER G,SELS B F,HOTTA J I,et al.Optical encoding of silver zeolite microcarriers[J].Advanced Materials,2010,22(9):957-960.

[6] EICHELBAUM M,RADEMANN K.Plasmonic enhancement or energy transfer On the luminescence of gold-,silver-,and lanthanide-doped silicate glasses and its potential for light-emitting devices[J].Advanced Functional Materials,2009,19(13):2045-2052.

[7] HEAVEN M W,DASS A,WHITE P S,et al.Crystal structure of the gold nanoparticle[N(C8H17)4][Au25(SCH2CH2Ph)18][J].Journal of the American Chemical Society,2008,130(12):3754-3755.

[8] ZHU M Z,AIKENS C M,HOLLANDER F J,et al.Correlating the crystal structure of a thiol-protected Au25 cluster and optical properties[J].Journal of the American Chemical Society,2008,130(18):5883-5885.

[9] AKOLA J,WALTER M,WHETTEN R L,et al.On the structure of thiolate-protected Au25[J].Journal of the American Chemical Society,2008,130(12):3756-3757.

[10] CHEN Y,LI W Y,WANG Y,et al.Cysteine-directed fluorescent gold nanoclusters for the sensing of pyrophosphate and alkaline phosphatase[J].Journal of Materials Chemistry C,2014,2(20):4080-4085.

[11] YUAN X,TAY Y,DOU X Y,et al.Glutathione-protected silver nanoclusters as cysteine-selective fluorometric and colorimetric probe[J].Analytical Chemistry,2013,85(3):1913-1919.

[12] PETTY J T,ZHENG J,HUD N V,et al.DNA-templated Ag nanocluster formation[J].Journal of the American Chemical Society,2004,126(16):5207-5212.

[13] GUO C L,IRUDAYARAJ J.Fluorescent Ag clusters via a protein-directed approach as a Hg(II) ion sensor[J].Analytical Chemistry,2011,83(8):2883-2889.

[14] ISHIDA T,NAGAOKA M,AKITA T,et al.Deposition of gold clusters on porous coordination polymers by solid grinding and their catalytic activity in aerobic oxidation of alcohols[J].Chemistry:A European Journal,2008,14(28):8456-8460.

[15] TSUNOYAMA H,SAKURAI H,NEGISHI Y,et al.Size-specific catalytic activity of polymer-stabilized gold nanoclusters for aerobic alcohol oxidation in water[J].Journal of the American Chemical Society,2005,127(26):9374-9375.

[16] BAO Y P,ZHONG C,VU D M,et al.Nanoparticle-free synthesis of fluorescent gold nanoclusters at physiological temperature[J].Journal of Physical Chemistry C,2007,111(33):12194-12198.

[17] SUN T,SEFF K.Silver clusters andchemistryin zeolites[J].Chemical Reviews,1994,94(4):857-870.

[18] DE CREMER G,ANTOKU Y,ROEFFAERS M B J,et al.Photoactivation of silver-exchanged zeolite A[J].Angewandte Chemie,2008,47(15):2813-2816.

[19] OZIN G A,HUGUES F.Silver atoms and small silver clusters stabilized in zeolite Y:optical spectroscopy[J].Journal of Physical Chemistry,1983,87(1):94-97.

[20] DE CREMER G,COUTIO-GONZALEZ E,ROEFFAERS M B J,et al.Characterization of fluorescence in heat-treated silver-exchanged zeolites[J].Journal of the American Chemical Society,2009,131(8):3049-3056.

[21] GELLENS L R,MORTIER W J,SCHOONHEYDT R A,et al.The nature of the charged silver clusters in dehydrated zeolites of type A[J].Journal of Physical Chemistry,1981,85(19):2783-2788.

[22] COUTINO-GONZALEZ E,ROEFFAERS M B J,DIEU B,et al.Determination and optimization of the luminescence external quantum efficiency of silver-clusters zeolite composites[J].Journal of Physical Chemistry C,2013,117(14):6998-7004.

[23] TIKHOMIROV V K,RODRGUEZ V D,KUZNETSOV A,et al.Preparation and luminescence of bulk oxyfluoride glasses doped with Ag nanoclusters[J].Optics Express,2010,18(21):22032-22040.

[24] KUZNETSOV A S,VELZQUEZ J J,TIKHOMIROV V K,et al.Quantum yield of luminescence of Ag nanoclusters dispersed within transparent bulk glass vs.glass composition and temperature[J].Applied Physics Letters,2012,101(25):251106.

[25] XIE J P,ZHENG Y G,YING J Y.Protein-directed synthesis of highly fluorescent gold nanoclusters[J].Journal of the American Chemical Society,2009,131(3):888-889.

[26] JIN R C.Quantum sized,thiolate-protected gold nanoclusters[J].Nanoscale,2010,2(3):343-362.

[27] LIN H,IMAKITA K,FUJII M,et al.New insights into the red luminescent bovine serum albumin conjugated gold nanospecies[J].Journal of Alloys and Compounds,2017,691:860-865.

[28] LI H W,YUE Y,LIU T Y,et al.Fluorescence-enhanced sensing mechanism of BSA-protected small gold-nanoclusters to silver(I) ions in aqueous solutions[J].Journal of Physical Chemistry C,2013,117(31):16159-16165.

王圣琼, 孙畅, 陶春先, 韩朝霞, 洪瑞金, 林辉, 张大伟. 牛血清蛋白-Au配合物及其复合物制备及表征[J]. 光学仪器, 2018, 40(1): 13. WANG Shengqiong, SUN Chang, TAO Chunxian, HAN Zhaoxia, HONNG Ruijin, LIN Hui, ZHANG Dawei. Preparation and characterization of bovine serum albumin(BSA)-Au[J]. Optical Instruments, 2018, 40(1): 13.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!