激光生物学报, 2016, 25 (6): 481, 网络出版: 2017-01-23  

生物医学光学中NADH荧光检测技术的发展

Development of NADH Fluorescence Detection Technology in Biomedical Optics
作者单位
1 西安交通大学 a.生命科学与技术学院综合实践训练第十小组
2 西安交通大学 b.生命科学与技术学院
3 西安交通大学 c.生物医学信息工程教育部重点实验室、生物医学分析技术与仪器研究所 陕西 西安 710049
摘要
NADH(还原型烟酰胺腺嘌呤二核苷酸)自1962年被发现至今已有五十多年了, 在此期间, 运用NADH及其相关技术进行医疗诊断与医学检测的方式不断发展, 领域也不断扩大。从当前的研究成果来看, NADH因为其独有的性质在现代医学诊断与检测领域发挥了极大的作用, 如实时、无损地监测脑部的活动; 清晰、有效地识别不同种类的脑组织; 抗细胞辐射损伤; 抗辐射诱导的细胞凋亡; 进行人体内的诸多平衡调节及人体生物钟调节等。本文将重点介绍在重要领域中的NADH研究和应用的突破。
Abstract
As NADH(reduced nicotinamide adenine dinucleotide) has been discovered over five decades since 1962, the using of NADH and its technique for medical diagnosis and medical testing methods emerge in an endless stream, the field is also expanding. From the current research results, NADH has played an important role in the field of modern medical diagnosis and detection because of its unique nature. Especially in the area of non-destructively monitoring brain activity in real-time,recognizing different kinds of brain tissue clearly and effectively, resisting cells radiation damage,resisting the apoptosis induced by radiation, regulating many balances in the human body and regulating human body biological clock.This paper will focus on the important areas of the breakthrough in NADH researches and adhibitions.
参考文献

[1] 李志豪, 刘建, 刘子恩, 等. 稀土上转换发光纳米探针用于潜指纹中可卡因的检测[J]. 分析科学学报, 2014, 30(5): 692-696.

    LI Zhihao, LIU Jian, LIU Zien, et al. Detection of exogenous compounds in latent fingerprints based on aptamers-functionalized upconversion nanoparticles[J]. Journal of Analytical Science, 2014, 30(5): 692-696.

[2] 张然星, 刘建彬, 谭延国. 几种化学发光检测系统测定血清胰岛素的临床效果评价[J]. 中华临床医师杂志(电子版), 2010, 4(11): 2218-2221.

    ZHANG Ranxing, LIU Jianbin, TAN Yanguo. Evaluation of clinical effects for different chemiluminescence immunoassay systems of serum insulin and C-peptide[J]. Chinese Journal of Laboratory Diagnosis, 2010, 4(11): 2218-2221.

[3] CHANCE B, COHEN P J, JOBSIS F F, et al. Intracellular oxidation-reduction states in vivo[J]. Science, 1962, 137(3529): 499-508.

[4] SHI H, SUN N, MAYEVSKY A, et al. Early identification of acute hypoxia based on brain NADH fluorescence and cerebral blood flow[J]. Journal of Innovative Optical Health Sciences, 2014, 7(2): 1450033-1-9.

[5] STIEFEL M F, SPIOTTA A, GRACIAS V H, et al. Reduced mortality rate in patients with severe traumatic brain injury treated with brain tissue oxygen monitoring[J]. Journal of Neurosurgery, 2005, 103(5): 805-811.

[6] SHI H, SUN N, MAYEVSKY A, et al. Preclinical evidence of mitochondrial nicotinamide adenine dinucleotide as an effective alarm parameter under hypoxia[J]. Journal of Biomedical Optics, 2014, 19(1): 17005.

[7] YU Q, HEIKAL A A. Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level[J]. Journal of Photochemistry & Photobiology B Biology, 2009, 95(1): 46-57.

[8] YASEEN M A, SAKADIC′ S, WU W, et al. In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH[J]. Biomedical Optics Express, 2013, 4(2): 307-321.

[9] DUCHEN M R. Mitochondria in health and disease: perspectives on a new mitochondrial biology[J]. Molecular Aspects of Medicine, 2004, 25(4): 365-451.

[10] DROZDOWICZ-TOMSIA K, ANWER A G, CAHILL M A, et al. Multiphoton fluorescence lifetime imaging microscopy reveals free-to-bound NADH ratio changes associated with metabolic inhibition[J]. Journal of Biomedical Optics, 2014, 19(8): 086016.

[11] BLACKER T S, MANN Z F, GALE J E, et al. Separating NADH and NADPH fluorescence in live cells and tissues using FLIM[J]. Nature Communications, 2014, 5: 3936.

[12] 刘发全, 邹杏坚, 陈永惠, 等. NADH对辐射后正常肝细胞株L02内ROS、Ca2+和pH水平的影响[J]. 肿瘤药学, 2015, 5(6): 425-429.

    LIU Faquan, ZOU Xingjian, CHEN Yonghui, et al. The effects of NADH on intracellular ROS, free Ca2+ and pH in L02 liver cells induced by X-ray irradiation[J]. Anti-tumor Pharmacy, 2015, 5(6): 425-429.

[13] 刘发全, 张积仁. NADH对X射线照射正常肝细胞L02后PARP和Caspase3活性的影响[J]. 黑龙江医学, 2015, 39(5): 468-469.

    LIU Faquan, ZHANG Jiren. Effect of NADH on the damage of L02 cells undergoing X-ray irradiation[J]. Heilongjiang Medical Journal, 2015, 39(5): 468-469.

[14] FRENCH S W. Chronic alcohol binging injures the liver and other organs by reducing NAD+ levels required for sirtuin’s deacetylase activity[J]. Experimental & Molecular Pathology, 2016, 100(2): 303-306.

[15] BITOUN J P, WEN Z T. Transcription factor Rex in regulation of pathophysiology in oral pathogens[J]. Molecular Oral Microbiology, 2015, 31(2): 115-124.

[16] XIANG Y L Y. Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets[J]. Nature Chemistry, 2011, 3(9): 697-703.

[17] SU J, XU J, CHEN Y, et al. Personal glucose sensor for point-of-care early cancer diagnosis[J]. Chemical Communication, 2012, 48(55): 6909-6911.

[18] YAN L, ZHU Z, ZOU Y, et al. Target-responsive “sweet” hydrogel with glucometer readout for portable and quantitative detection of non-glucose targets[J]. Journal of the American Chemical Society, 2013, 135(10): 3748-3751.

[19] ZHANG J, XIANG Y, WANG M, et al. Dose-dependent response of personal glucose meters to nicotinamide coenzymes: applications to point-of-care diagnostics of many non-glucose targets in a single step[J]. Angew Chem Int Ed Engl, 2016, 55(2): 732-736.

[20] LAN T, ZHANG J, LU Y. Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health[J]. Biotechnology Advances, 2016, 34(3): 331-341.

[21] OMAR F S, DURAISAMY N, RAMESH K, et al. Conducting polymer and its composite materials based electrochemical sensor for Nicotinamide Adenine Dinucleotide (NADH)[J]. Biosensors & Bioelectronics, 2016, 79: 763-775.

张瑞, 黄玉广, 裴应玫, 党昱东, 张知, 张建保, 张镇西. 生物医学光学中NADH荧光检测技术的发展[J]. 激光生物学报, 2016, 25(6): 481. ZHANG Rui, HUANG Yuguang, PEI Yingmei, DANG Yudong, ZHANG Zhi, ZHANG Jianbao, ZHANG Zhenxi. Development of NADH Fluorescence Detection Technology in Biomedical Optics[J]. Acta Laser Biology Sinica, 2016, 25(6): 481.

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