Synthesis of biocompatible near infrared fluorescence <sub>2</sub> quantum dot and its application in bioimaging

Xin Zhang; Yueqing Gu; Haiyan Chen

doi:doi:10.1142/s1793545813500594

Journal of Innovative Optical Health Sciences, 2014, 7 (3): 1350059, Published Online: Jan. 10, 2019

Synthesis of biocompatible near infrared fluorescence ₂ quantum dot and its application in bioimaging

论文大纲

Xin Zhang Yueqing Gu ^*Haiyan Chen

Author Affiliations

Department of Biomedical Engineering School of Life Science and Technology China Pharmaceutical University, 24 Tongjia Lane Gulou District, Nanjing 210009, P. R. China

Near infrared fluorescence quantum dot toxicity bioimaging

Abstract

Near infrared (NIR) emitting quantum dots (QDs) is a promising candidate for biomedical imaging in living tissues. However, the biomedical application of NIR QDs was not satisfactory due to their toxicity. ₂ QDs was reported to have negligible toxicity in organisms. Therefore, the appropriate narrow bandgap and low toxicity of ₂ QDs facilitated them to be a promising contrast agent for fluorescence imaging. Here, a low toxicity, stable and highly luminescent NIR ₂ QDs were prepared by one-step aqueous method using 2-mercaptopropionic acid (MPA) as the coating layers. Emission wavelength of ₂ QDs could be tuned between 780 and 950 nm. MTT assay results indicated that there was no significant biotoxicty for ₂ QDs. These NIR QDs exhibited excellent biocompatibility in tumor cells. The cellular uptake and localization of ₂ QDs was studied using laser confocal scanning microscopy. ₂ QDs were effectively internalized by the cells. Therefore, ₂ QDs, acting as a novel fluorescence probe, has promising potential in biolabeling, deep tissue imaging, diagnostics and photodynamic therapy.

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Xin Zhang, Yueqing Gu, Haiyan Chen. Synthesis of biocompatible near infrared fluorescence ₂ quantum dot and its application in bioimaging[J]. Journal of Innovative Optical Health Sciences, 2014, 7(3): 1350059.

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