基于加权平均法的活体藻类三维荧光标准光谱构建

针对活体荧光光谱不稳定引起的蓝藻门活体藻类定量误差问题，以实验室培养的4种类6个生长期的48个蓝藻样品为研究对象，通过测量样品叶绿素a和藻蓝蛋白的含量，结合藻类活体三维荧光光谱，研究了不同藻种种类、生长期和生长环境下蓝藻细胞色素组成和色素荧光效率的差异；定量分析不同条件对藻类活体荧光光谱不稳定性的影响，获得了不同条件下的光谱不稳定性权重谱；在此基础上，构建基于加权平均方法的蓝藻门活体藻类加权荧光光谱；比较了加权荧光光谱与不同条件下归一化荧光光谱对样品集的测量结果。结果表明：加权荧光光谱能有效降低荧光测量法对藻种种类、生长期、生长环境的依赖性，提高蓝藻门叶绿素浓度的测量准确性；测量结果的相对误差为0.1%~30.4%，平均相对误差为12.8%，相对误差最大可降低104.1%。

Abstract

Forty-eight samples belonging to four common species of Cyanophyta are studied in order to reduce the chlorophyll a concentration measurement errors caused by unstable fluorescence spectra in vivo. We analyze the effects of algae species, growing period, and growing environment on the photosynthetic pigment composition and fluorescence efficiency by measuring the content of chlorophyll a and phycocyanin, as well as the three-dimensional fluorescence spectra of Cyanophyta in vivo. And we obtain the weight spectra by analyzing the spectral instability under different habitat conditions. The weight fluorescence spectrum of Cyanophyta in vivo is established based on the weighted average method. The measurement results that respectively obtained by the weight spectrum and different normalized spectra are compared, and the comparisons indicate that the weight spectrum can significantly reduce the dependence of fluorescence method on algae species, growing period, and growing environment, and thus increase the accuracy of chlorophyll a concentration of Cyanophyta. The relative error of the weight spectrum is 0.1%-30.4%, with a mean relative error of 12.8%. The maximum relative error of anabaena can be reduced by 104.1% in the weight spectrum.

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