一种中药荧光指纹图谱的光谱图像构建方法

利用液晶光谱仪通过光谱成像法对黄柏和人参总皂甙进行了荧光光谱图像检测，采用滤波器与像素点定位联用的方法提取有效像素点，获取它们的荧光光谱图像，绘制了相应的中药荧光指纹图谱.结合欧氏距离的判定方法，将滤波器与像素点定位联用法、像素点优选法和背景差分算法所得的荧光指纹图谱进行比较，三种方法的差异由小到大的排序为滤波器与像素点定位联用法，背景差分算法，像素点优选法.说明滤波器与像素点定位联用法所得荧光指纹图谱稳定性最好，谱线最优.实验结果表明该方法可以用于构建中药荧光指纹图谱，从而提出了一种新的用于中药指纹图谱构建中提取有效像素点的可行方法.

Abstract

In order to improve the stability of Traditional Chinese Medicine′s fluorescence fingerprint spectra of constructing images and data processing efficiency, Liquid Crystal Spectrometer was used to detect spectral image of Cortex Phellodendri and Total Saponins of Panax Ginseng. Effective pixels were extracted using the combination of filter and localizing pixels means, besides, their pixels′ spectral images were obtained to draw Traditional Chinese Medicine′s fluorescent fingerprints correspondingly. Considering their Euclidean distance, when compared the three methods, the combination of filter and localizing pixels means has the best fluorescence fingerprint and spectral stability, followed by the background differencing, then is the optimization of pixels. By experimental results, it is revealed that spectral stability by this way is better and can be used to build Traditional Chinese Medicine′s fluorescent fingerprints. Based on these points, a new approach used for collecting effective pixels during Traditional Chinese Medicine′s fingerprints construction is proposed.

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