红细胞内吞银包金纳米颗粒的实时表面增强喇曼研究

研究了结晶紫包裹的银包金纳米粒子进入红细胞的实时过程.利用激光光镊喇曼技术每隔20 s通过光镊囚禁红细胞并收集该细胞及邻近溶液的喇曼光谱,抽取光谱中具代表性的特征峰来观察其强度随时间的变化.结果表明:从囚禁的红细胞中收集到的光谱包括了归属红细胞与结晶紫的特征峰.红细胞的光谱特征峰1 001、1 128、1 213 cm－1和结晶紫的光谱特征峰915、1 177、1 389、1 586、1 619 cm－1的强度随着时间增加,表明在红细胞与纳米粒子共培养的过程中,纳米粒子在红细胞中累积,并引起红细胞信号增强.分析红细胞与其邻近溶液的光谱差,发现归属结晶紫的光谱特征峰913、1 179、1 586 cm－1随时间呈类余弦的变化,表明红细胞内的结晶紫包裹的银包金纳米粒子含量先升高后降低再升高.通过计算得到纳米粒子开始进入红细胞的时间范围及进入的速度、被溶酶体降解的速度.研究表明表面增强喇曼技术为研究外物进入细胞提供了新的实验方法和思路.

Abstract

Tracking the process which erythrocyte endocytoses gold core-silver shell nanoparticles wrapped in crystal violet with laser optical tweezers raman technique was studied.The erythrocytes were imprisoned by optical tweezers every 20 s, the raman spectra of erythrocytes and the adjacent solutions were collected.Results show that the collected spectra of erythrocytes include the characteristic peaks of erythrocyte and crystal violet.The intensity of peaks belonging to erythrocyte of 1 001,1 128,1 213 cm－1 and which belonging to crystal violet of 915,1 177,1 370,1 586,1 619 cm－1increase over time,which show that in the process of co-culture with erythrocytes and nanoparticles,nanoparticles could increase the signal of erythrocyte and be accumulated in erythrocytes.By analyzing the difference of spectra value between erythrocytes and its adjacent solutions,it finds that spectral characteristic peaks belonging to crystal violet of 913,1 179,1 586 cm－1 changing like cosine with time,which suggested that the nanoparticles in erythrocyte induced after first increased,and then increased again.The time range where nanoparticles began to enter erythrocyte,the entering speed and the rate of lysosome degrading nanoparticles wrapped in crystal were caculated.This study shows that surface-enhanced raman spectroscopy can provide a new idea and an experimental method for the study of foreign objects into to cells.

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张枝芝, 林漫漫, 张泽森, 徐斌, 姚辉璐, 刘军贤. 红细胞内吞银包金纳米颗粒的实时表面增强喇曼研究[J]. 光子学报, 2015, 44(6): 0630005. ZHANG Zhi-zhi, LIN Man-man, ZHANG Ze-sen, XU Bin, YAO Hui-lu, LIU Jun-xian. Real-time Study on Erythrocyte Endocytosing Ag@AuNPs by Surface-enhanced Raman Scattering[J]. ACTA PHOTONICA SINICA, 2015, 44(6): 0630005.

红细胞内吞银包金纳米颗粒的实时表面增强喇曼研究

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