激光光谱技术在呼吸气体分析中的发展与未来 
下载： 1479次
Breath Analysis Using Laser Spectroscopy Techniques: Development and Future
1 中国科学院苏州生物医学工程技术研究所, 江苏 苏州 215163
2 中国科学院生物医学检验技术重点实验室, 江苏 苏州 215163
3 中国医学科学院生物医学工程研究所, 天津 300192
4 美国密西西比州立大学, 美国 密西西比 MS 39762
图 & 表
图 1. CRDS技术示意图
Fig. 1. Illustration of CRDS technique
下载图片 查看原文
图 2. 第一代CRDS呼吸丙酮分析仪
Fig. 2. The CRDS breath acetone analyzer I
下载图片 查看原文
图 3. 第一代CRDS呼吸丙酮分析仪线性度响应
Fig. 3. Linear response of the CRDS breath acetone analyzer I
下载图片 查看原文
图 4. 第一代CRDS呼吸丙酮分析仪的重复性
Fig. 4. The reproducibility of the CRDS breath acetone analyzer I
下载图片 查看原文
图 5. 第二代CRDS呼吸丙酮分析仪
Fig. 5. The CRDS breath acetone analyzer II
下载图片 查看原文
图 6. 三类受试者的呼吸丙酮平均浓度
Fig. 6. Mean breath acetone concentrations of three subjects
下载图片 查看原文
图 7. 排除酮症外的20名I型糖尿病受试者的个体呼吸丙酮浓度和平均血糖浓度的线性关系
Fig. 7. Linear relation of the breath acetone concentrationwith the mean blood glucose level in 20 TD1 subjects with no ketoacidosis
下载图片 查看原文
表 1已建立的生物标记物及其对应的生理症状[7]
Table1. Established breath biomarker and their physiological symptoms[7]
| Breath biomarker | Metabolic disorder /disease | Classification |
|---|
| Acetaldehyde (CH3CHO) | Alcoholism, liver related diseases, lung cancer | Disease | | Acetone [OC(CH3)2] | | Lung cancer, diabetes, dietary fat losses,congestive heart failure, brain seizure | | Ammonia (NH3) | | Renal diseases, asthma | | Carbondisulphide (CS2) | | Schizophrenia, coronary, artery disease | | Carbonyl sulfide (OCS) | | Liver related diseases | | Hydrogen peroxide (H2O2) | | Asthma | | Methylated (CH3OH) | | Nervous system disorder | | Methyl nitrate (CH3NO3) | | Hyperglycemia intype 1 diabetes | | Nitrogen monoxide (NO) | | Asthma | | Nitrotyrosine (C9H10N2O5) | | Asthma, bronchiectasis, hypertension, rhinitis, lung disease | | Pyridine (C5H5N) | | Periodontal disease | | Sulfur compounds | | Hepatic diseases and malodor, lung cancer | | Butane (C4H10) | Tumor marker in lung cancer | | Metabolicdisorder | | Carbon monoxide (CO) | Oxidative stress, respiratory infection, anaemias | | | | Carbon dioxide (CO2)(13C-isotopes) | Helicobacter pylor, oxidative stress | | | | Ethane (C2H6) | Vitamin E deficiency in children,lipid peroxidation, oxidative stress | | | | Ethanol (C2H5OH) | Production of gut bacteria | | | | Ethylene (C2H4) | Lipid peroxidation, ultra violet radiation damage of skin | | | | H/D isotope | Body water | | | | Hydrogen (H2) | Indigestion in infants, intestinal upset, colonic fermentation | | | | Hydrogen cyanide (HCN) | Pseudomon asaeruginosa in children affected with cystic fibrosis | | | | 8-isoprostane | Oxidative stress | | | | Isoprene | Blood cholesterol | | | | Methane (CH4) | Intestinal problems, colonic fermentation | | | | Oxygen (O2) | Respiration | | | | Pentane (C5H12) | Peroxidation of lipids, liver diseases, schizophrenia,breast cancer, rheumatoid arthritis | | |
|
查看原文
表 2主要生物标记物的光谱指纹以及采用的光谱技术和检测极限[7]
Table2. Spectral fingerprints, laser spectroscopic technique, and detection limit of major biomarkers[7]
| Breath biomarkers(chemical formula) | Spectral fingerprints(UV-MIR) /μm | Laser spectroscopictechnique | Detection limit | Researchers |
|---|
| Acetaldehyde (CH3CHO) | 5.79 | TDLAS | 8×10-8 | Kamat, et al | | Acetone [OC(CH3)2] | 0.266 | CRDS | 5×10-8 | Wang, et al | | Ammonia (NH3) | 9-10.7 | PAS | 1×10-4 | Narasimhan, et al | | NH3 | 11.0 | TDLAS | 1×10-6 | Lachish, et al | | NH3 | 10.0 | TDLAS | 3×10-9 | Manne, et al | | NH3 | 10.3 | TDLAS | 5×10-9 | Moskalenko, et al | | NH3 | 1.5 | OFC-CEAS | 4×10-6 | Thorpe, et al | | Carbon dioxideand 13C-isotopes | 4.23 | PAS | 7×10-9 | Herpen, et al | | (CO2 & 13CO2/12CO2) | | | | | | CO2 | 1.6 | CRDS | 3×10-6 | Crosson, et al | | CO2 | 1.59 | TDLAS | 1×10-4 | Weldon, et al | | CO2 | 4.9 | TDLAS | 5×10-7 | Moskalenko, et al | | CO2 | 4.9 | CALOS | (3.778±0.004)% | Halmer, et al | | CO2 | 5.2 | ICOS | - | McCurdy, et al | | 13CO2/12CO2 | 1.6 | CRDS | Precision, 0.2‰ | Crosson, et al | | 13CO2/12CO2 | 1.6 | OFC-CEAS | Precision,4.1‰ | Thorpe, et al | | Carbon monoxide (CO) | 1.6 | OFC-CEAS | 9×10-7 | Thorpe, et al | | CO | 4.6 | TDLAS | 5×10-7 | Moskalenko, et al | | CO | 4.88 | TDLAS | - | Lee, et al | | Carbonyl sulfide (OCS) | 4.86 | TDLAS | 1.2×10-9 | Wysocki, et al | | OCS | 4.86 | TDLAS with QC laser | 3×10-8 | Roller, et al | | OCS | 4.9 | CALOS | 4.38×10-10 | Halmer, et al | | Ethane (C2H6) | 3.4 | OA-ICOS | 1.2×10-10 | Parameswaran, et al | | C2H6 | 3.4 | TDLAS | 1×10-10 | Skeldon, et al | | C2H6 | 3.3 | CALOS | 2.7×10-10 | Halmer, et al | | C2H6 | 3.0 | CALOS | 1×10-10 | Dahnke, et al | | C2H6 | 2.6-4.0 | CALOS | 5×10-10 | VonBasum, et al | | C2H6 | 3.4 | TDLAS | (0-12)×10-8 | Patterson, et al | | C2H6 | 3.3 | PAS | - | Puiu, et al | | Ethylene (C2H4) | 10.5 | PAS | - | Puiu, et al | | C2H4 | 9.2-10.8 | PAS | - | Dumitras, et al | | Methane (CH4) | 3.35 | TDLAS | 5×10-7 | Moskalenko, et al | | Nitrogen monoxide (NO) | 5.2 | ICOS | 1×10-9 | Silva, et al | | NO | 5.2 | TDLAS | 2×10-9 | Namjou, et al | | NO | 5.2 | TDLAS | 3×10-9 | Menzle, et al | | NO | 5.2 | CRDS | 7×10-10 | Kosterev, et al | | NO | 5.2 | TDLAS | 1.5×10-9 | Roller, et al | | NO | 5.2 | ICOS | 4×10-10 | McCurdy, et al |
|
查看原文
姜琛昱, 孙美秀, 李迎新, 王储记. 激光光谱技术在呼吸气体分析中的发展与未来[J]. 中国激光, 2018, 45(2): 0207015. Jiang Chenyu, Sun Meixiu, Li Yingxin, Wang Chuji. Breath Analysis Using Laser Spectroscopy Techniques: Development and Future[J]. Chinese Journal of Lasers, 2018, 45(2): 0207015.
PDF全文
