Advanced Photonics, 2020, 2 (3): 036005, Published Online: Jun. 3, 2020
Frequency-domain diagonal extension imaging 
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Fig. 1. An illustration of the FDDE microscopy. (a) Illustration of the sampling interval of an image sensor with a rectangular pixel in the horizontal and diagonal directions. (b) The frequency domain of an image sensor and the OTF of an imaging system in undersampled digital imaging. The green-dash rectangle is the frequency domain of the microscopic image. The yellow-dot circle is the OTF of an imaging system. The green-line rectangle is the frequency domain of undersampled digital imaging. D and H denote the diagonal and horizontal directions, respectively. The spatial domain image of (b) is shown in the upper-right corner. (c) The optical setup of FDDE LFM. (d) Illustration of the frequency stitching algorithm of FDDE.
Fig. 2. LFM imaging with different directions of the image sensor. (a) and (b) The reconstructed hologram images of LFM in the horizontal and diagonal directions, respectively. (c) and (d) The frequency domain of the reconstructed images in (a) and (b), respectively. The dotted rectangle marked in (d) represents the effective frequency boundaries in (c). (e) and (f) The line profiles (element 3 of group 8) marked in (a) and (b), respectively. The red dotted line profile in (f) is the same location in the image that filtered the high frequency out of the yellow rectangle, as illustrated by the inset in (d). The yellow arrows indicate the direction of the sample.
Fig. 3. Demonstration of FDDE imaging with a mouse skin sample. (a) The FDDE LFM image of the mouse skin sample. (b) An enlarged view of the region marked in (a). (c) LFM images. (c1), (c2), and (c3) are the same area as (c4) in the three-phase images with different orientations. The arrows in the upper-right corner correspond to the direction of the sample in the experiment. The three arrows indicate the FDDE image. In addition, (c2) and (c3) and (d2) and (d3) are rotated back to the same direction as in (c1) and (d1), respectively, for a comparison. The line profile in (c4) is marked between the arrows. The inset in (c4) is imaged with a
Fig. 4. Analysis of FDDE imaging with a blood smear specimen. (a) The FDDE image with the blood smear specimen. (b) An enlarged view of the region marked in (a). (c1)–(c3) The conventional LFM of different angles, and (c4) is the LFM with FDDE. The thick arrows in the upper-right corner correspond to the direction of the sample in the experiment in (c1)–(c3). The three arrows indicate the combined FDDE image in (c4). (d) The bright-field image of the same area, presented as the ground truth. (e) The line profile from (c1)–(c4) marked in (c4).
Shan Jiang, Meiling Guan, Jiamin Wu, Guocheng Fang, Xinzhu Xu, Dayong Jin, Zhen Liu, Kebin Shi, Fan Bai, Shu Wang, Peng Xi. Frequency-domain diagonal extension imaging[J]. Advanced Photonics, 2020, 2(3): 036005.
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