Structured illumination microscopy (SIM) achieves super-resolution (SR) by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction. The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain, it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary, besides, the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts. Here, we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets, and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets (the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function (OTF)). Experiments on reconstructing raw datasets including nonbiological, biological, and simulated samples demonstrate that our method has SR capability, high reconstruction speed, and high robustness to aberration and noise.

为解决在激光直写系统中利用数字微镜器件(DMD)无法对纵向像素数大于768的灰度图像进行滚动显示的问题,文章在其控制电路的数据传输、存储及显示驱动技术方面开展了研究。利用FPGA中丰富的逻辑资源实现了图像数据传输方式的改进,通过移位寄存器对数据进行拆分和位宽转换。提出间隔存储方法实现大尺寸灰度图像数据的存储,基于此方法可实现灰度图像滚动显示时的数据读取。同时,省去在上位机中进行位平面拆分和图像分割的预处理步骤,简化了上位机操作流程并提高了系统数据传输效率。实验结果表明,该系统能够以419.6Hz的刷新率对大尺寸灰度图像进行滚动显示。

非均匀性是影响数字微镜器件（Digital Micro-mirror Device, DMD）红外场景产生器投射红外场景质量的主要因素之一, DMD红外场景产生器必须经过非均匀性校正才能满足复杂环境下红外成像设备内场仿真试验的应用要求。给出了 DMD红外场景产生器的非均匀性校正流程；提出了一种更适合于现有测试条件的变尺度稀疏网格非均匀性测量方法；采用线性化和分段校正进行离线数据处理；采用“在线查表法”进行实时非均匀性校正。仿真结果表明: 经过变尺度稀疏网格测试法及非均匀性实时校正算法, 可将非均匀性降低至 0.5%左右, 显著提高了 DMD红外场景产生器的红外场景仿真质量。

There is an ongoing technological revolution in the field of biomedical instruments. Consequently, high performance healthcare devices have led to remarkable economic developments in the medical hardware industry. Until now, nearly all optical bio-imaging systems are based on the 2-dimensional imaging chip architecture. In fact, recent developments in digital micromirror devices (DMDs) are gradually making their way from conventional optical projection displays into biomedical instruments. As an ultrahigh-speed spatial light modulator, the DMD may offer a range of new applications including real-time biomedical sensing or imaging, as well as orientation tracking and targeted screening. Given its short history, the use of DMD in biomedical and healthcare instruments has emerged only within the past decade. In this paper, we first provide an overview by summarizing all reported cases found in the literature. We then critically analyze the general pros and cons of using DMD, specifically in terms of response speed, stability, accuracy, repeatability, robustness, and degree of automation, in relation to the performance outcome of the designated instrument. Particularly, we shall focus our discussion on the use of Micro-Electro-Mechanical System (MEMS)-based devices in a set of representative instruments including the surface plasmon resonance biosensor, optical microscopes, Raman spectrometers, ophthalmoscopes, and the micro stereolithographic system. Finally, the prospects of using the DMD approach in biomedical or healthcare systems and possible next generation DMD-based biomedical devices are presented.