Zebrafish is an important animal model, which is used to study development, pathology, and genetic research. The zebrafish skin model is widely used in cutaneous research, and angiogenesis is critical for cutaneous wound healing. However, limited by the penetration depth, the available optical methods are difficult to describe the internal skin structure and the connection of blood vessels between the skin and subcutaneous tissue. By a homemade high-resolution polarization-sensitive optical coherence tomography (PS-OCT) system, we imaged the polarization contrast of zebrafish skin and the zebrafish skin vasculature with optical coherence tomography angiography (OCTA). Based on these OCT images, the spatial distribution of the zebrafish skin vasculature was described. Furthermore, we monitored the healing process of zebrafish cutaneous wounds. We think the highresolution PS-OCT system will be a promising tool in studying cutaneous models of zebrafish.

偏振敏感光学相干层析术可对生物双折射组织进行功能成像,然后通过分析相位延迟就可得到生物组织的信息。分析了单一入射偏振态对双折射相位延迟准确度的影响。首先提出了系统的理论模型,然后进行MATLAB模拟分析,最后通过实验进一步验证了理论模型的结果。通过MATLAB得到的图像以及实验结果可以看出:在样品具有确定的相位延迟量以及确定的光轴方向时,当线偏振入射光的偏振方向与光轴夹角为0°或者90°时,或当正椭圆入射光的偏振方向与光轴夹角为45°时,相位延迟的测量误差最小。

偏振频域光学相干层析成像(OCT)中图像质量受散斑噪声影响较大,散斑噪声会使图像细节变模糊,降低图像清晰度。针对此问题,提出了一种分光谱降低偏振频域OCT散斑噪声的方法。该方法将系统的全光谱信号分为多个光谱信号,对每个分立的光谱信号进行窗函数滤波,单独进行常规数据处理,然后将处理后的各个分光谱进行平均合成,达到降低散斑噪声的目的。利用Matlab进行仿真,同时搭建实验系统对离体生物样品鸡胸肉进行检测。实验结果表明,该方法可有效降低散斑噪声,提高偏振图像质量。

主要研究了基于单探测器的偏振光学相干层析技术(PS-OCT),探索并验证其定性检测生物组织双折射性质的能力。论述了单探测器PS-OCT系统检测组织双折射性质的理论,并利用Monte Carlo方法模拟了双折射对Stokes矢量分布的影响。在此基础上,搭建了单探测器PS-OCT系统,系统的纵向分辨率可达10 μm。利用该系统对牛软骨组织进行成像实验,获得反映牛软骨双折射性质的组织图像。理论和实验结果均表明,基于单探测器的PS-OCT系统可以检测组织双折射性质的变化,能够应用于医学诊断组织病变。