Temporary spinal cord stimulation (tSCS) can effectively reduce the pain and severity of postherpetic neuralgia (PHN). However, there are no effective and objective methods for predicting the effects of tSCS on PHN. Laser speckle contrast imaging (LSCI) is frequently used in neurology to evaluate the effectiveness of treatment. To assess the accuracy of LSCI in predicting the impact of tSCS on PHN, 14 adult patients receiving tSCS treatments for spinal nerve-innervated (C6-T2) PHN participated in this observational study. Visual analog scale (VAS) assessments and LSCI blood flow images of the fingers were recorded after the tSCS procedure. The results showed that the VAS scores of all patients decreased significantly. Moreover, the blood flow index (BFI) values were significantly higher than they were before the procedure. Increased blood flow and pain alleviation were positively correlated. The findings indicated that spinal nerve PHN (C6-T2) was significantly reduced by tSCS. Pain alleviation by tSCS was positively correlated with increased blood flow in the hand. The effect of tSCS on PHN may thus be predicted using an independent and consistent indicator such as LSCI.

激光散斑会严重影响图像的质量，降低图像的对比度和分辨率，为了抑制激光投影散斑，基于人眼视觉暂留特性和漫散射体散斑抑制原理，搭建了一套基于运动循环胶质溶液的散斑抑制装置，该装置体积小，并能有效抑制散斑，实现散斑对比度低于人眼观测阈值 5%的抑制效果，光的透过率可达 65%以上，同时该装置比起振动扩散器，在降低噪声及价格上具有明显的优势，具有一定的实用推广价值。

Laser speckle contrast imaging (LSCI) is a powerful tool for monitoring blood flow changes in tissue or vessels in vivo, but its applications are limited by shallow penetration depth under reflective imaging configuration. The traditional LSCI setup has been used in transmissive imaging for depth extension up to 2lt–3lt (lt is the transport mean free path), but the blood flow estimation is biased due to the depth uncertainty in large depth of field (DOF) images. In this study, we propose a transmissive multifocal LSCI for depth-resolved blood flow in thick tissue, further extending the transmissive LSCI for tissue thickness up to 12lt. The limited-DOF imaging system is applied to the multifocal acquisition, and the depth of the vessel is estimated using a robust visibility parameter Vr in the coherent domain. The accuracy and linearity of depth estimation are tested by Monte Carlo simulations. Based on the proposed method, the model of contrast analysis resolving the depth information is established and verified in a phantom experiment. We demonstrated its effectiveness in acquiring depth-resolved vessel structures and flow dynamics in in vivo imaging of chick embryos.

将扩散干涉光谱（iDWS）技术与光外差检测方法和扩散散斑衬比分析方法相结合，提出了一种扩散相干散斑成像检测方法，并搭建了实验系统。该系统能有效地提高无创检测局部脑血流量（rCBF）的信噪比和检测精度，仿体流速实验结果表明，其相对血流指数与实际流速具有较好的线性度，在源-探距离为6~12 mm范围内线性相关系数均值为0.9881±0.0005。该检测方法可以区分不同目标区域的流速变化，管径为4.8 mm时相对误差为2.04%，结合血管管径测量方法可实现流速和流量的有效监测。通过在体实验和袖带加压实验，证明系统可以检测到活体rCBF的流速信息，且在有效测量流速范围内有较好的检测准确度。

激光散斑对比成像(LSCI)是一种以宽视场方式监测血流速度的非扫描光学成像技术。LSCI技术具有高时间-空间分辨率、快速实时成像、非接触式、仪器结构简单、无需造影剂等优势。本文简要介绍了LSCI的基本原理，概述了反射式LSCI和透射式LSCI两种结构，综述了LSCI在皮肤血流、大脑皮层和视网膜血流等生物医学应用中的最新研究进展，并对其发展前景做了进一步展望，为血流监测提供理论依据和实践指导。

提出一种手动旋转双散射片的散斑噪声抑制方法，以一动一静的工作模式获得多幅独立的散斑场，通过多幅再现像叠加实现散斑的抑制。通过理论分析一动一静双散射片和单散射片的时间相关函数，证明一动一静双散射片的去相关速率明显高于单散射片，具有更好的散斑抑制效果。实验上分别进行了不同目数单散射片和双散射片、相同目数单散射片和双散射片之间的对比研究。从散斑对比度结果可以看出，散射片目数越多，再现像叠加数量越多，散斑抑制效果越好，并且双散射片的散斑抑制效果要优于单散射片。以相位光栅为样本，采用一动一静双散射片方式验证了所提方法的可行性，该方法获得了更好的成像效果。

Reduced nicotinamide adenine dinucleotide (NADH) plays a crucial role in many biochemical reactions in human metabolism. In this work, a flow-mediated skin fluorescence (FMSF)-postocclusion reactive hyperaemia (PORH) system was developed for noninvasive and in vivo measurement of NADH fluorescence and its real-time dynamical changes in human skin tissue. The real-time dynamical changes of NADH fluorescence were analyzed with the changes of skin blood flow measured by laser speckle contrast imaging (LSCI) experiments simultaneously with FMSFPORH measurements, which suggests that the dynamical changes of NADH fluorescence would be mainly correlated with the intrinsic changes of NADH level in the skin tissue. In addition, Monte Carlo simulations were applied to understand the impact of optical property changes on the dynamical changes of NADH fluorescence during the PORH process, which further supports that the dynamical changes of NADH fluorescence measured in our system would be intrinsic changes of NADH level in the skin tissue.

激光散斑是阻碍激光投影显示技术发展的一大瓶颈。基于复合散斑衬比度分析法，对影响激光投影系统中复合散斑衬比度的参数进行了分析，即统计独立散斑图样个数N和探测器成像镜头在投影屏幕上的分辨基元面积内的散射光波的相干面元的数量M。可通过调节投影镜头和成像镜头的数值孔径、投影距离及观测距离等参数改变N和M，从而达到改善激光投影系统中散斑现象的目的。并在简化的投影系统中，就随机相位片上照射光斑面积、照明系统数值孔径对激光投影成像中散斑衬比度的影响进行了系统实验分析。实验结果表明，在投影镜头尺寸一定的基础上，适当增加照射在随机相位片上光斑的面积，可有效降低散射屏幕上二次散斑图样的衬比度，且投影镜头数值孔径的减小会增加散斑现象，这为激光投影系统的小型化提出了一大挑战。该结论可为以旋转随机相位片抑制激光投影成像中的散斑提供系统设计指导。