Author Affiliations
Abstract
1 Institute of Biomedical Technologies Privolzhsky Research Medical University 10/1 Minina Square, Nizhny Novgorod 603005, Russia
2 Laboratory of Laser Nanomodification of Materials Institute of Applied Physics Russian Academy of Sciences 46 Ulyanova Street, Nizhny Novgorod 603950, Russia
3 Institute of Photon Technologies of Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences 2 Pionerskaya Street, Moscow, Troitsk 108840, Russia
4 Department of Innovative Program Institute of Applied Physics Russian Academy of Sciences 46 Ulyanova Street, Nizhny Novgorod 603950, Russia
5 Department of Urology Privolzhsky Research Medical University 10/1 Minina Square, Nizhny Novgorod 603005, Russia
The bactericidal effect of laser radiation with a quartz fiber-based transmission system with a strong absorption coating converter against bacteria associated with urological stones has been studied. Gram-negative rod Escherichia coli and the Gram-positive coccus Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium were used in this study. Each bacterial species was treated by continuous-wave near infrared laser coupled with bare fiber tip or strongly absorption coating fiber tip. After treatment, the temperature of bacterial suspension was measured. In addition, the temperature distribution was analyzed. It has been shown that using laser with a strongly absorption coating fiber tip results in significant bactericidal effect. The decrease of the amount of E. coli and S. epidermidis was 100% after treatment with an output power of 6W of radiation at a wavelength of 0.97 _m for 40 s. Number of S. aureus and Ent. faecium colony-forming unit was reduced to 70% after same exposure. The peak temperature of bacterial suspension was 86_C after treatment by laser with a strongly absorption coating fiber tip. Laser with a strongly absorption coating fiber tip provides large-scale hydrodynamic flows directed away from the fiber tip. The laser with a strongly absorption coating fiber tip has bactericidal effect. The main role is associated with the effect of high temperature, which, in the form of flow in a liquid medium, affects bacteria.
Laser strongly absorbing coating bacteria bactericidal effect laser-induced hydrodynamic Journal of Innovative Optical Health Sciences
2018, 11(5): 1850029
Author Affiliations
Abstract
1 Institute of Biomedical Technologies,Nizhny Novgorod State Medical Academy,10/1 Minin and Pozharsky Sq., Nizhny Novgorod 603005, Russia
2 Lobachevsky State University of Nizhni Novgorod,23 Gagarin Avenue BLDG 2, Nizhny Novgorod 603950, Russia
3 Nizhny Novgorod State Medical Academy 10/1 Minin and Pozharsky Sq., Nizhny Novgorod 603005, Russia
4 Institute of Applied Physics, Russian Academy of Sciences 46 Ul'yanov Street, Nizhny Novgorod 603950, Russia
5 Institute on Laser and Information Technologies Russian Academy of Sciences 2 Pionerskaya St., Troitsk 142092, Russia
In this paper, we consider a method of laser resection using the silica glass core from which the cladding layer has been removed as the cutting part of a laser scalpel. An absorbing layer coating the silica fiber tip markedly alters its biotissue cutting characteristics. The results of histological studies of skin after exposure to a laser scalpel with and without a strongly absorbing coating (SAC) at a wavelength of 0.97 μm show that resection using a coated scalpel is more sparing. When an uncoated scalpel was used, skin injury was more apparent in both its surface spread and the depth of structural damage, resulting in poorer tissue regeneration.
Laser surgery silica fibers strongly absorbing coating histology Journal of Innovative Optical Health Sciences
2016, 9(5): 1650011
