1 天津大学 精密仪器与光电子工程学院,天津 300072
2 天津市生物医学检测技术与仪器重点实验室,天津 300072
基于电子倍增电荷耦合器件探测器搭建了面向小鼠活体非接触式测量的荧光扩散光层析成像系统,为了避免复杂的焦点矫正计算并降低逆问题的病态性,提出一种新的电荷耦合器件平板探测器信息提取及扩展方法,并根据信息提取方法发展了非接触式荧光扩散光层析成像图像重建算法.通过大量仿体荧光扩散光层析成像实验,证明当提取的信息对应于圆柱域投影的1/2、且将信息提取域分解为3个子域时重建可获得最好的效果.制备了皮下植入荧光目标体的活体小鼠进行荧光扩散光层析成像实验,与显微CT成像结果的对比表明：搭建的系统结合所发展的图像重建算法能够较好地重建出活体小鼠内荧光目标体的位置和浓度,有望应用到小鼠肿瘤模型的荧光层析成像中.
荧光层析成像 信息提取及扩展方法 小动物 皮下肿瘤成像 小动物成像荧光标记 Fluorescence diffuse optical tomography Data-Extraction-and-Extension-Method(DEEM) Small animal Subcutaneous tumor imaging Fluorescent markers to small-animal imaging 光子学报
2014, 43(10): 1011002
1 天津大学 精密仪器与光电子工程学院,天津 300072
2 天津市生物医学检测技术与仪器重点实验室,天津 300072
在Newton-Raphson逆模型框架下，发展了基于稳态辐射传输方程光子输运正模型和代数重建技术的扩散光学层析图像重建算法，并针对小尺寸组织体内部吸收和散射系数同时重建情况下的标准代数重建技术算法进行了改进，数值结果表明改进后的代数重建技术算法重建图像效果优于标准代数重建技术算法.
扩散光学层析 辐射传输方程 逆问题 小动物成像 Diffuse optical tomography Radiative transfer equation Inverse problem Small-animal imaging
Author Affiliations
Abstract
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source. A high sensitivity cooled charge-coupled device (CCD) camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images. The laser beam performs fast raster scanning using a dual-axis galvanometric scanner. The accuracy of the laser spot position at the source window is within +-200 \mum. Based on the phantom experimental results, the spatial resolution is less than 1.7 mm, and the relative quantitation error is about 10%. In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.
荧光分子层析成像 小动物成像 170.6960 Tomography 170.6280 Spectroscopy, fluorescence and luminescence 170.0110 Imaging systems Chinese Optics Letters
2010, 8(11): 1075
