荧光素汞在碱性条件下具有很强的荧光,H2S能与荧光素汞结合,使其荧光猝灭,据此建立了一种荧光法测定大鼠肠灌流液中H2S含量的方法.在0.1 mol·L-1 NaOH溶液中,以Na2S作为H2S供体,荧光素汞浓度为5.0×10-5 mol·L-1,Na2S浓度为1.0×10-5 mol·L-1时,以498 nm为激发波长,在522 nm处测定此二元体系的荧光强度.结果表明,在4.0×10-7~2.0×10-6 mol·L-1范围内,H2S浓度与荧光强度的下降程度呈良好的负相关性,r=0.998 0,精密度实验RSD=4.59%(n=7),检出限3.5×10-8 mol·L-1,样品中H2S含量分别为1.01×10-6和1.15×10-6 mol·L-1,加标回收率为95.8%~101.0%.该方法操作简单,灵敏度高,稳定性好,可准确测定肠灌流液中H2S含量,为内源性H2S的测定提供了依据.

Under alkaline conditions,Fluorescein mercury has strong fluorescence,however,when it met S2-,its fluorescence would quench,in view of the above,a fluorescence method for determination of H2S in biological samples was established.In the 0.1 mol·L-1 NaOH dilution,when the concentration of fluorescein Mercury and Na2S was 5.0×10-5 and 1.0×10-5 mol·L-1 respectively,the fluorescence intensity of system was determined at 522 nm.The results showed that,at the range of 4.0×10-7~2.0×10-6 mol·L-1,the concentration decreasing of H2S and fluorescence intensity had good linear relationship,r=0.998 0,the RSD of precision test was 4.59% (n=7),the detection limit was 3.5×10-8 mol·L-1,the content of H2S in the sample were 1.01×10-6 and 1.15×10-6 mol·L-1,and the recovery rate was 95.8%~101.0%,the method has the advantages of simple operation,high sensitivity,good selectivity,can accurately determine of H2S in intestinal perfused solution,and provides the basis for the determination of endogenous H2S.