光动力法制备抗小鼠H22肝癌的肿瘤疫苗

张红雨; 马文江; 邹朝晖; 高卫平; 薛志孝; 李迎新

中国激光, 2008, 35 (4): 631, 网络出版: 2008-04-21

光动力法制备抗小鼠H22肝癌的肿瘤疫苗

Generation of Antitumor Vaccines for H22 Tumor on Mouse Using Photodynamic Therap

论文大纲

张红雨 ^1,*马文江 ²邹朝晖 ¹高卫平 ¹薛志孝 ¹李迎新 ¹

作者单位

¹ 天津医科大学生物医学工程系激光医学实验室, 天津 300070

² 天津医科大学眼科中心检验科, 天津 300070

医用光学光动力疗法肿瘤疫苗免疫调节昆明鼠 medical optics photodynamic therapy tumor vaccine immune regulation Kunming mouse

摘要

研究了光动力疗法(PDT)制备的抗小鼠H22肝癌肿瘤疫苗的抗瘤效应。将昆明鼠60只,随机分为2组,每组30只。实验组取6～12周龄的昆明鼠背部皮下接种光动力疗法产生的疫苗,每3天注射一次,每次注射50 μL(相当于3×105个细胞),连续两周。隔一周于第22天注射H22肿瘤细胞悬液0.1 mL(1×106个细胞); 对照组:每周每次注射50 μL生理盐水,连续两周。隔一周于第22天注射H22肿瘤细胞悬液0.1 mL(1×106个细胞)。比较两组的抑瘤率、生存率以及两组之间免疫学的相关指标。结果表明,实验组小鼠具有显著的抑瘤效果,抑瘤率、生存率较对照组有显著提高。实验组肿瘤抑瘤率最高可达60%且长期有效,100天生存率达56%。说明光动力疗法产生的抗小鼠H22肝癌疫苗可以有效地抑制肿瘤生长,提高荷瘤小鼠的生存率,具有明显的抗瘤效应。该方法可能成为一种辅助性治疗肿瘤的手段而应用于临床。

Abstract

To test the contribution of the direct effects of photodynamic therapy (PDT) on tumor cells, we examined the immunogenicity of PDT-generated murine tumor cell lysates in a preclinical vaccine model. Sixty Kunming mice (H22 tumor host) were divided into two groups randomly and equally. Six to twelve-week-old Kunming mice were vaccinated intradermally on the right shoulder with 50 μL lysates (3×105 cell equivalents) for experimental group or medium control for control group every three days during two weeks. The mice rested a week and then inoculated on the flank with 1×106 tumor cells harvested from exponentially growing cultures. And then we compared antitumor rate, survial rate and relevant indicators of immunology between two groups. PDT vaccines could inhibit the tumor growth rate compared to the contrast group. The tumor inhibition rate of PDT vaccines group was 60% and long-term available. The survival rate of PDT vaccines group at 100 day was 56% which was significantly higher than contrast group. Our studies suggest that PDT-generated vaccines could effectively inhibit tumor growth, improve survival rate of mice in experimental group, and enhance antitumor immune response significantly. PDT-generated vaccines may have well clinical potential as an adjuvant therapy.

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张红雨, 马文江, 邹朝晖, 高卫平, 薛志孝, 李迎新. 光动力法制备抗小鼠H22肝癌的肿瘤疫苗[J]. 中国激光, 2008, 35(4): 631. Zhang Hongyu, Ma Wenjiang, Zou Zhaohui, Gao Weiping, Xue Zhixiao, Li Yingxin. Generation of Antitumor Vaccines for H22 Tumor on Mouse Using Photodynamic Therap[J]. Chinese Journal of Lasers, 2008, 35(4): 631.

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