发光学报, 2014, 35 (6): 649, 网络出版: 2014-06-10   

量子点尺寸对量子点和金纳米粒子之间的荧光共振能量转移的影响

Influence of The Size of Quantum Dot on Fluorescence Resonance Energy Transfer between Quantum Dots and Au Nanoparticles
作者单位
发光学及应用国家重点实验室 中国科学院长春光学精密机械与物理研究所, 吉林 长春130033
摘要
量子点(QDs)所具有的一元激发多元发射的特点预示着QDs在高通量的多元量化生物信息处理领域具有应用前景。采用柠檬酸钠直接还原法制备了粒径为15 nm的Au纳米粒子, 利用静电自组装的方法构建了Au与QDs的荧光共振能量转移(FRET)系统。采用该系统研究了QDs的尺寸变化对FRET效率的影响, 获得了FRET效率和给体与受体之间交叠积分的关系。结果证明, QDs是构建多元均相免疫检测技术的一个有效给体。
Abstract
The most promising application of quantum dots (QDs) is to high throughput quantitative biological information processing because of their advantages with the different emissions for single excitation wavelength. A facile method is presented to integrate Au nanoparticles with QDs for constructing QDs@Au composite nanostructures by electrostatic interaction, in which the Au nanoparticles are synthesized with sodium citrate. Based on this composite structure, the influence of the size of QDs on the FRET efficiency is researched. These results demonstrate that the QDs are effective donors in multi-homogeneous immune-assay based on FRET.
参考文献

[1] Fster T. Intermolecular energy migration and fluorescence [J]. Ann. Phys., 1948, 2:55-75.

[2] He Y F, Lu M L, Cao J, et al. Manipulating protein conformations by single-molecule AFM-FRET nanoscopy [J]. ACS Nano, 2012, 6(2):1221-1229.

[3] Sapsford K E, Granek J, Deschamps J R, et al. Monitoring botulinum neurotoxin an activity with peptide-functionalized quantum dot resonance energy transfer sensors [J]. ACS Nano, 2011, 5(4):2687-2699.

[4] Zhang C Y, Yeh H C, Kuroki M T, et al. Single-quantum-dot-based DNA nanosensor [J]. Nat. Mater., 2005, 4(11):826-831.

[5] Zhang H, Xu T, Li C W, et al. A microfluidic device with microbead array for sensitive virus detection and genotyping using quantum dots as fluorescence labels [J]. Biosens. Bioelectron., 2010, 25(11):2402-2407.

[6] Zeng Q H, Zhang Y L, Liu X M, et al. Multiple homogeneous immunoassays based on a quantum dots-gold nanorods FRET nanoplatform [J]. Chem. Commun., 2012, 48(12):1781-1783.

[7] Xia Y S, Song L, Zhu C Q. Turn-on and near-infrared fluorescent sensing for 2,4,6-trinitrotoluene based on hybrid (gold nanorod)-(quantum dot) assembly [J]. Anal. Chem., 2011, 83(4):1401-1407.

[8] Saha K, Agasti S S, Kim C, et al. Gold nanoparticle in chemical and biological sensing [J]. Chem. Rev., 2012, 112(5):2739-2779.

[9] Tu L P, Kong X G. Studies on the constructure based on luminescence resonant energy transfer between NaYF4∶Yb,Er @SiO2 nanostructure as donors and gold nanoparticle as acceptors [J]. Chin. J. Lumin.(发光学报), 2013, 34(2):149-153 (in Chinese).

[10] Zhang Y L, Kong X G, Xue B, et al. Effect of aggregation of Ag nanoparticles suspended in aqueous solution on surface enhanced Raman scattering [J]. Chin. J. Lumin.(发光学报), 2014, 35(2):263-267 (in Chinese).

[11] Xia L, Kong X G, Liu X M, et al. An upconversion nanoparticle-zinc phthalocyanine based nanophotosensitizer for photodynamic therapy [J]. Biomaterials, 2014, 35(13):4146-4156.

李晓坤, 张友林, 孔祥贵. 量子点尺寸对量子点和金纳米粒子之间的荧光共振能量转移的影响[J]. 发光学报, 2014, 35(6): 649. LI Xiao-kun, ZHANG You-lin, KONG Xiang-gui. Influence of The Size of Quantum Dot on Fluorescence Resonance Energy Transfer between Quantum Dots and Au Nanoparticles[J]. Chinese Journal of Luminescence, 2014, 35(6): 649.

本文已被 1 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!