辣椒红素分子体外氧化防御反应UV-Vis及FTIR光谱特征分析

郑文宇; 丁筑红; 刘海

doi:doi:10.3964/j.issn.1000-0593(2014)02-0358-06

光谱学与光谱分析, 2014, 34 (2): 358, 网络出版: 2015-01-13

辣椒红素分子体外氧化防御反应UV-Vis及FTIR光谱特征分析

Spectral Characterization for Capsanthin Molecule with in Vitro Oxidative Defense Reaction

论文大纲

郑文宇 ^*丁筑红刘海

作者单位

贵州大学酿酒与食品工程学院，贵州省农畜产品贮藏与加工重点实验室，贵州贵阳550025

辣椒红素紫外-可见光谱红外光谱光谱特征 Capsanthin UV-Vis Spectra FTIR Spectra Spectral characterization

摘要

采用紫外-可见光谱(UV-Vis)和傅里叶变换红外光谱(FTIR)法分析辣椒红素在外源活性氧·O-2， H2O2和·OH以及在POD， CAT和LOX防御体系中的结构变化特征。结果表明，外源活性氧处理后辣椒红素最大紫外-可见光吸收峰均出现蓝移现象，其中·O-2和·OH处理后产物FTIR谱峰数减少，峰强变弱，峰形变宽；与色素分子中的羰基和环外烯基发生氧化反应生成烷基和羟基， ·OH对色素分子CC具有加成作用； H2O2处理后色素特征峰和强势峰均向低波数位移，产物中含环氧醚基团；在H2O2+CAT/POD防御体系中辣椒红素UV-Vis和FTIR光谱特征无明显变化；在亚油酸+LOX体系中色素分子结构发生断裂，产物不含羰基。可见活性氧处理及在LOX底物体系中，辣椒红素分子反应前后UV-Vis和FTIR等光谱特征发生明显变化，色素分子中长烯链断裂，大共轭体系缩短或被破坏，分子中的共轭双键和羰基等发色基团改变，最终生成醇类或醚类等小分子无色物质；而防御酶CAT和POD能减少活性氧对辣椒红素分子的破坏作用。研究结果丰富完善了辣椒色素理论，同时为其开发利用提供了有价值的技术参考依据。

Abstract

The changes of UV-Vis and FTIR spectroscopic properties for capsanthin before and after reaction with exogenous superoxide anion(·O-2), hydrogen peroxide(H2O2) and hydroxyl radical(·OH), catalase(CAT), peroxidase(POD) and lipoxygenase(LOX) were explored. The results showed that, the UV-Vis spectral absorption of capsanthin treated with reactive oxygen species had a blue-shift. At the same time, the FTIR spectra changed significantly. The number of FTIR spectral peaks reduced and theFTIR strength weakened for capsanthin molecule treated with ·O-2 and ·OH. The characteristic and strong peaks moved to shorter wavelengths when treated with H2O2. And LOX caused breakage of capsanthin molecule and reduction of peak number or groups without carbonyl. Exogenous H2O2+CAT or H2O2+POD treatment could not affect the UV-Vis and FTIR spectra significantly. So ROS could cause oxidative degradation of capsanthin and destroy chromophoric groups such as carbon-carbon double bond and carbonyl, then grow colorless alcohols. Hence ROS and LOX should transforms the conjugate system of capsanthin molecules, while CAT and POD could protect the capsanthin.

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郑文宇, 丁筑红, 刘海. 辣椒红素分子体外氧化防御反应UV-Vis及FTIR光谱特征分析[J]. 光谱学与光谱分析, 2014, 34(2): 358. ZHENG Wen-yu, DING Zhu-hong, LIU Hai. Spectral Characterization for Capsanthin Molecule with in Vitro Oxidative Defense Reaction[J]. Spectroscopy and Spectral Analysis, 2014, 34(2): 358.

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